Sugarcane Mosaic Virus (SCMV) causes one of the most severe virus diseases in maize worldwide, resulting in reduced grain and forage yield in susceptible cultivars. In this study, two association panels consisting of 94 inbred lines each, from China and the U.S., were characterized for resistance to two isolates: SCMV-Seehausen and SCMV-BJ. The population structure of both association panels wa...

The lectin-binding properties and glycosidase sensitivities of the virion glycoproteins of primate cytomegaloviruses (CMVs) were examined. Three simian CMV (SCMV) strains, including Colburn, and four human CMV (HCMV) strains were compared. Their proteins were separated in denaturing polyacrylamide gels and electrotransferred onto nitrocellulose, and the glycosylated species were visualized with...

Symptom development of a plant viral disease is a result of molecular interactions between the virus and its host plant; thus, the elucidation of specific interactions is a prerequisite to reveal the mechanism of viral pathogenesis. Here, we show that the chloroplast precursor of ferredoxin-5 (Fd V) from maize (Zea mays) interacts with the multifunctional HC-Pro protein of sugar cane mosaic vir...

Sugarcane mosaic potyvirus (SCMV) UP-isolate was antigenically similar to SCMV-A, SCMVMDB and two SCMV isolates from Thailand. The results were confirmed either by DAC-ELISA, EBIA, DIBA and ISEM independently or in combination. No reaction was evident with antiserum specific for other members of the Sugarcane mosaic potyvirus subgroup or any other potyviruses tested. The peptide profiling of a ...

Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investigated in two sugarcane varieties contrasting to SCMV resistance, when challenged with a severe virus strain. Healthy plants derived from meristem tip tissue culture were mechanical...

Previously, sugarcane mosaic virus (SCMV) was developed as a vector for transient expression of heterologous genes in Zea mays (maize). Here, we show that SCMV can also be applied virus-induced gene silencing (VIGS) endogenous maize genes. Comparison sense and antisense VIGS constructs targeting phytoene desaturase (PDS) showed resulted greater reduction expression. In time course after infecti...

A sensitive duplex-immunocapture-RT-PCR (D-IC-RT-PCR) technique was developed for detection and discrimination of taxonomically distinct Sugarcane streak mosaic virus (SCSMV) and Sugarcane mosaic virus (SCMV) that naturally infect sugarcane. D-IC-RT-PCR was performed using polyclonal antisera for capture of virions. Oligo 5'-d(T)18(AGC)-3' as a common reverse primer for both viruses and virus s...

Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...

Maize dwarf mosaic and sugarcane mosaic, the diseases incited by Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV), are among the most important maize viral diseases worldwide (28). The two viruses are related members of the genus Potyvirus that are nonpersistently transmitted by aphids (16) and can be mechanically transmitted experimentally. They are differentiated from one ano...

two hundred and thirty one leaf samples of virus infected and mosaic and as well dwarf mosaic symptoms showing maize plants from various corn fields in tehran province were collected during the growing season 2003. by performing serological tests of das-elisa, diba and tpia, using scmv, mdmv, srmv and jgmv antisera, only scmv was detected in samples, with all samples reacting strongly to the sc...