The effects of starvation and subsequent re-feeding on compensatory growth performance, blood serum metabolites and IGF-ImRNA expression in liver and muscle were investigated in juvenile Persian sturgeon. Growth indices including body weight, SGR, CF, and HSI significantly decreased after starvation. However, after re-feeding sturgeons that were starved for 1 week reached the same weight as the...

Serum starvation is a common cell culture procedure for increasing cellular response to insulin, though the mechanism for the serum starvation effect is not understood. We hypothesized that factors known to potentiate insulin action [e.g., AMP-activated protein kinase (AMPK) and p38] or to be involved in insulin signaling leading to glucose transport [e.g., Akt, PKCζ, AS160, and ataxia telangie...

Serum starvation is one of the most frequently performed procedures in molecular biology and there are literally thousands of research papers reporting its use. In fact, this method has become so ingrained in certain areas of research that reports often simply state that cells were serum starved without providing any factual details as to how the procedure was carried out. Even so, we quite obv...

background: the human leukocyte antigen (hla) matching between organ donor and recipient is an acceptable strategy in clinical transplantation since 1964. however, in bone marrow transplantation, finding matched donors is often problematic. thus new method for down regulation of hla can be an alternative strategy to solve this problem. objective: to examine the effect of serum starvation on hla...

Serum starvation is a typical way for inducing tumor cell apoptosis and stress. Asymmetric dimethylarginine (ADMA) is an endogenous metabolite. Our previous study reveals the plasma ADMA level is elevated in colon cancer patients, which can attenuate serum starvation-induced apoptosis in LoVo cells. In current study, we evaluated the effects of ADMA on gene expression and metabolism in serum-st...

Background: The human leukocyte antigen (HLA) matching between organ donor and recipient is an acceptable strategy in clinical transplantation since 1964. However, in bone marrow transplantation, finding matched donors is often problematic. Thus new method for down regulation of HLA can be an alternative strategy to solve this problem. Objective: To examine the effect of serum starvation on HLA...

Cellular excretion of inflammatory cytokines is an important experimental read-out in a wide range of molecular biology fields. The addition of serum to growth media provides the optimal growing conditions for most cell types. When studying the effect of bacteria and bacterial products on these cells serum starvation is often performed as a standard procedure [1] to avoid unwanted stimulation b...

Parathyroid hormone (PTH) acts on bone and kidneys by binding to PTH/PTH-related protein (PTHrP) receptors and regulating calcium (Ca) and phosphorus (P) homeostasis. PTH/PTHrP receptor mRNA was expressed at high levels in PTH target tissues such as the kidneys and bone including the calvaria, femur, and tibia. Because short-term starvation influences Ca and P ion homeostasis, we measured chang...

Borrelia burgdorferi, the causative agent of Lyme disease, is transmitted by the tick Ixodes scapularis. A 2.9-kb fragment containing a putative spoT gene was isolated from B. burgdorferi genomic DNA by PCR amplification and cloned into a pBAD24 vector. The cloned gene complemented Escherichia coli mutant strain CF1693, which contains deletions of both the relA and spoT genes. The spoT gene in ...