امرالله مصطفی زاده

[ 1 ] - بررسی میزان القاء اینترلوکین-1 بتا (IL-1β)، فاکتور نکروزدهنده توموری (TNF-α) و مایلوپراکسیداز (MPO) پالپ دندان رت هایپرگلیسمیک توسط مینرال تری اکساید اگریگیت (MTA) و سمان غنی شده با کلسیم (CEM)

مقدمه: ‌هدف این مطالعه بررسی میزان بیان اینترلوکین-1 بتا (IL-1β)، فاکتور نکروزدهنده توموری (TNF-α) و مایلوپراکسیداز (MPO) بعد از استفاده از MTA))Mineral Trioxide Aggregate  و Calcium-Enriched Mixture (CEM) به عنوان پالپ کپ در رت‏های دیابتی ‏بود. مواد و روش‏ها: از مدل حیوانی با القای دیابت با استفاده از Streptozotocin (تزریق داخل صفاقی به صورت mg/kg 65) استفاده شد. 34 رت به دو گروه دیابتی و سا...

[ 2 ] - Stable Down-Regulation of HLA Class-I by Serum Starvation in Human PBMCs

Background: The human leukocyte antigen (HLA) matching between organ donor and recipient is an acceptable strategy in clinical transplantation since 1964. However, in bone marrow transplantation, finding matched donors is often problematic. Thus new method for down regulation of HLA can be an alternative strategy to solve this problem. Objective: To examine the effect of serum starvation on HLA...

[ 3 ] - Both immune hormone IL-10 and parathormone tend to increase in serum of old osteoporotic people independently of 1, 25 dihydroxy vitamin D3

Background: In this study, we determined the serum levels of IL-17A and IL-10 in context with 1, 2 dihydroxy vitamin D3, parathormone and Ca2+/Pi ions to investigate their pathological or protective roles respectively in bone metabolism. Methods: The bone mineral density (BMD) was determined for 1203 participants using energy X-ray absorptiometry. Subjects with a history of diseases and usin...

[ 4 ] - Peripheral blood lymphocytes are able to maintain their viability and basic function in normal urine

Background: Similar to inflammatory cells, peripheral blood mononuclear cells (PBMCs) can also infiltrate in to kidney and urinary tracts and subsequently excreted by urine. In this study we determined the viability rate and response to phytohemagglutinin-A (PHA) of human PBMCs in normal urine. Methods: A number of 1×106 ficoll-hypaque isolated PBMCs were dispensed in 1 ml normal urine...

[ 5 ] - Histopathologic Responses of the Dental Pulp to Calcium-Enriched Mixture (CEM) and Mineral Trioxide Aggregate (MTA) in Diabetic and Non-Diabetic Rats

Diabetes mellitus is a chronic disease which affects the healing ability of the pulp and periodontium. The aim of the present study was to assess the histopathologic response of dental pulp to pulp capping using MTA or CEM cement in diabetic rats. Thirty two Wistar male rats aged between 8 and 10 weeks (weight: 200-250g) were divided into two groups of diabetic (n=16) and healthy (n=16) animals...

[ 6 ] - Human Fibroblast Switches to Anaerobic Metabolic Pathway in Response to Serum Starvation: A Mimic of Warburg Effect

Fibroblasts could be considered as connective tissue cells that are morphologically heterogeneous with diverse functions depending on their location and activity. These cells play critical role in health and disease such as cancer and wound by Production of collagen, fibronectin, cytokines and growth factors. Absence of insulin and other growth factors in serum deprivation condition and similar...

[ 7 ] - Total Phenolics and Flavonoids Contents of Aqueous Extract of Stinging nettle and In Vitro Antiproliferative Effect on Hela and BT- 474 cell lines

Phenolic compounds including flavonoids and phenolic acids are plants secondary metabolites. Due to their ability to act as antioxidant agents, there is a growing interest to use those components in traditional medicine for cancer prevention or treatment. The aim of this study was to measure the amounts of total phenolics and flavonoids as well as anti-proliferative effect of aqueous extract of...

[ 8 ] - Spontaneous Mesenchymal to Epithelial Like Tissue Transition (MET) in a Long Term Human Skin Culture

In an attempt to isolate multipotent stem cells from foreskin in a long-term culture, we encountered an interesting phenomenon which was the conversion of the fibroblast dominant condition to epithelial-like tissue formation. However, the basic mechanism(s) which may be involved in this conversion is not clear. This study was designed to evaluate the cells protein secretion activity and examine...

[ 9 ] - Systemic effects of starved fibroblasts culture supernatant on immunosuppressed rats treated with cancer stem cells

[ 10 ] - Effect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method

Introduction: Bone marrow and immune cell transplants are a common method of treating some blood diseases around the world. However, due to the malfunction of the immune system, its use is not always effective. In this study, we evaluated the potential of culture stress in serum-free medium in regulating the immune system. Materials and Methods: Spleen immune cells were isolated from Balb/C and...