sperm dna is known to contribute one half of the genomic material to the offspring. the integrity of sperm dna is important in fertilization, embryonic and fetal development, and postnatal child well being. the nature has created multiple barriers that allow only the fittest sperm to reach and fertilize an oocyte. however, assisted reproductive techniques (art), like ivf and icsi, may allow spe...

Sperm DNA damage has a negative impact on pregnancy rates following assisted reproduction treatment (ART). The aim of the present study was to examine the relationship between sperm DNA fragmentation and live-birth rates after IVF and intracytoplasmic sperm injection (ICSI). The alkaline Comet assay was employed to measure sperm DNA fragmentation in native semen and in spermatozoa following den...

background: oxidative stress in reproductive system leads to sperm dna damage and sperm membrane lipid peroxidation and may play an important role in the pathogenesis of male infertility, especially in idiopathic cases. antioxidants such as carotenoids function against free radical damages. objective : the aim of this study was to determine the levels of lycopene, beta-carotene and retinol in s...

Background: Sperm chromatin insufficiencies leading to low sperm count and quality, infertility and transmission of chromosomal microdeletion and aneuploidies to next generations can be due to exposure to environmental pollutions, chemicals and natural or manmade ionizing radiation. In this project which has continued for more than 10 years and is unique in many technical aspects in Iran and in...

evidence supports the involvement of nitric oxide (no) in a varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. however, low concentration of no is essential in biology and physiology of spermatozoa, but high amounts of no is toxic and has negative effects on sperm functions. on the other hand, it is established t...

Background: Although sperm selection according to morphologic and motility characteristic, made to some what the fertility successful, but sperm’s normal morphology cannot demonstrate sperm DNA competence. Since the health of sperm DNA is affecting the results of assisted reproductive technology; the aim of this study is to evaluate semen parameters measured by CASA with sperm chromatin abnorma...

Background: The pathophysiology of the varicocele has received considerable study, both in humans and in animal models. The sperm DNA damage and related oxidative stress have been identified as significant cause of male infertility. Present study was designed to evaluate the testicular nitric oxide (NO) generation, lipid and carbonyl groups (CGs) peroxidation and to analyze related sperm DNA da...