research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

introduction: the purpose of this study was to evaluate the effect of different doses of retinoic acid on resumption of meiosis, in vitro maturation of oocytes and resulting embryo development in mice. materials and methods: germinal vesicle of nmri female mouse (6-8 weeks old) oocytes were collected from ovaries and cultured in maturation medium mem-α contained: 100 mliu/ml rfsh + 7.5 iu/ml hc...

a gas permeation model (coupling model) has been developed which has the flexibility to be used for different membrane module configurations. the aim of this work is to predict the performance of a single stage gas separation process using membranes and provide a comprehensive description of process parameters like flow rates, composition, stage cut and stream pressure. the significant feature ...

Background: While it is possible to routinely cryopreserve embryos from several mammalian species, the cryopreservation of embryos has largely been limited by their high sensitivity to chilling injury. Many factors such as the stage of embryonic development, cryoprotectant toxicity, the composition of the vitrification solution and cooling and warming rates can influence survival of embryos aft...

technology development projects, unlike many other kinds of projects don’t have pre-defined goals or clear implementation methods at the beginning of the project. among other uncertainties that any project could have, technology development projects have a high degree of uncertainty in the definition of project. for this reason, conventional project management methods such as those provided in ...

low water consumption in autumn sugar beet sowing is an important strategy for sugar beet production, especially in arid regions like iran. for the development of a new monogerm triploid hybrid called sharif which is bolting tolerant and suitable for autumn sowing in khuzestan province, a breeding program was started in 1994 and continued for16 years. this program had two stages; the first stag...