This report described Sarcocystis-like merozoites in peripheral blood smear of a woman with mild fever, rigor and musculoskeletal pain. Extracellular organisms with a pale blue cytoplasm and one or two nuclei were seen in her blood smear stained with Giemsa. Sarcocystis-like can be considered as a possible cause of some idiopathic febrile diseases.

BACKGROUND Cutaneous Leishmaniasis (CL) is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize Leishmania species among the negative direct smears obtained from skin ulcer...

The detection and identification of intestinal helminths were studied retrospectively by comparing the Formalin concentration technique with the trichrome-stained smear technique. A total of 3,997 stool samples from 1,570 patients were examined by both methods. Of the 3,997 samples, 31% (1,239 of 3,997) contained helminths or protozoans or both. A total of 11% (434 of 3,997) of the samples repr...

BACKGROUND In countries with high tuberculosis (TB) burden, there is urgent need for rapid, large-scale screening to detect smear-positive patients. We developed a computer-aided whole smear screening system that focuses in real-time, captures images and provides diagnostic grading, for both bright-field and fluorescence microscopy for detection of acid-fast-bacilli (AFB) from respiratory speci...

Many laboratory workers prefer the rhodamine-auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rhodamine-auramine stain. A total of 782 primary smears were evaluated blindly by...

A rapid test for diagnosis of malaria based on acridine orange staining of centrifuged blood samples in a microhaematocrit tube (QBC) was compared with Leishman stained thin peripheral blood smear in 287 samples. Malaria was diagnosed in 44 patients by Leishman staining technique and in 65 patients by QBC method. The QBC method allowed detection of an additional 21 cases. Thus the prevalence ra...

this report described sarcocystis-like merozoites in peripheral blood smear of a woman with mild fever, rigor and musculoskeletal pain. extracellular organisms with a pale blue cytoplasm and one or two nuclei were seen in her blood smear stained with giemsa. sarcocystis-like can be considered as a possible cause of some idiopathic febrile diseases.

In the original communication of Churchman 1 on the selective bactericidal action of gentian violet, published two years ago, the suggestion was made that similar studies should be carried out on the effect of the dye on growing tissue. This communication contains the report s of work undertaken in that direction. The work was primarily undertaken to settle two questions raised by the observati...

ABSTRACT Objective: to assess Pap smear performance in bacterial vaginosis diagnosis women of childbearing age. Method: a cross-sectional, retrospective study carried out health unit city the interior São Paulo. The included information from 1,173 who underwent collection and Gram-stained vaginal smears (reference standard test for diagnosis) analysis based on Nugent score, January 2013 June 20...

The present study is conducted to demonstrate the value of direct smear examinations ( Gram stain and wet mount ) of vaginal discharge from infected female patients. The diagnosis of bacterial vaginosis and other infections were carried out by direct gram stained smear and wet mount preparations of vaginal discharge in 1004 cases of various infections affecting these patients. Gram stain was a ...