Analysis of synthetic gene regulatory circuits can provide insight into circuit behavior and evolution. An alternative approach is to modify a naturally occurring circuit, by using genetic methods to select functional circuits and evolve their properties. We have applied this approach to the circuitry of phage . This phage grows lytically, forms stable lysogens, and can switch from this regulat...

|Test generation using deterministic fault-oriented algorithms is highly complex and time-consuming. New approaches are needed to augment the existing techniques, both to reduce execution time and to improve fault coverage. In this work, we describe a genetic algorithm (GA) framework for sequential circuit test generation. The GA evolves candidate test vectors and sequences, using a fault simul...

We show how to build an inductorless version of the classic Chua’s circuit. The goal is to build Chua’s circuit quickly and easily. To this end, only off-the-shelf components are used. A suitable inductor for Chua’s circuit is often hard to procure. Here, the inductor is replaced by a single op-amp synthetic inductor circuit. The synthetic inductor is novel in the sense that it is not a gyrator...

Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of pa...

The development and progress in synthetic biology has been remarkable. Although still in its infancy, synthetic biology has achieved much during the past decade. Improvements in genetic circuit design have increased the potential for clinical applicability of synthetic biology research. What began as simple transcriptional gene switches has rapidly developed into a variety of complex regulatory...

A basic assumption underlying synthetic biology is that analysis of genetic circuit elements, such as regulatory proteins and promoters, can be used to understand and predict the behavior of circuits containing those elements. To test this assumption, we used time-lapse fluorescence microscopy to quantitatively analyze two autoregulatory negative feedback circuits. By measuring the gene regulat...

in the present study, a model of a large lithium polymer (li-po) battery for use in the simulation of hybrid electric vehicles (hevs) is developed. to attain this goal, an equivalent circuit (ec) consisting of a series resistor and two rc parallel networks is considered. the accuracy and the response time of the model for use in an hev simulator are studied. the battery parameters identificatio...