an agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain ...

background: sugarcane is a monocotyledonous crop that is cultivated in the tropical and subtropical regions of the world. one of the most important criteria, influencing the efficiency of the sugarcane transformation is known to be related to physical and biological factors during the transformation procedure. objectives: the objective of this research was to study the response of callus induc...

gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out gene transfer to plants performs to evaluate the transient and permanent gene expression. transient expression is quick, easy and simple and is not influenced by position effect compare ...

One of the most efficient mechanisms by which plants protect themselves from invading virusesis the specific RNA-dependent silencing pathway termed post-transcriptional gene silencing(PTGS). In this mechanism, resistance to a virus is engineered through the expression of asegment of the virus genomein transgenic plants. Potato VirusY (PVY) is one of the mostdamaging viruses of potato, infecting...

Objective(s): Experimental myocardial infarction triggers secretion of Stromal cell-derived factor1 and lead to increase in the expression of its receptor "CXCR4" on the surface of various cells. The aim of this study was to evaluate the expression pattern of CXCR4 in peripheral blood cells following time-course permanent and temporary ischemia in rats. Materials and Methods: Fourteen male Wist...

Background: Pilot and large-scale production of recombinant proteins requires the presence of stable clones capable of producing large quantities of recombinant proteins. Not only the process of selecting stable clones is time consuming, but also the continuous culturing of clones in large-scale production may cause loss of incoming plasmid and recombinant genes. Thus, considering the advanceme...

It is possible to use transgenic plants, as bioreactors, for the production of recombinant inexpensive chemicals and drug components. Transient gene expression is an appropriate alternative to stable transformation because it allows an inexpensive and rapid method for expression of recombinant proteins in plant tissues. In recent years, plant viral vectors have been increasingly developed as su...

Background: The potential of plants, as a safe and eukaryotic system, is considered in the production of recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (PTGS) response in this new technology. The use of viral suppressors of gene silencing can prevent PTGS and improve transient expression level ...

bacillus anthracis as a spore-forming bacterium is the causative agent for anthrax. three proteins from b. anthracis including protective antigen (pa), lethal factor (lf) and edema factor (ef) are causative factors of this bacteria. to produce plant-based vaccine, fusion protein of pa 4 domain and lf 1 domain via furin cleavage site joined to subunit b vibrio cholera toxin (ctb) as an inducer o...