the aim of this study was to determine the occurrence of enterotoxigenic and enteroaggregative escherichia coli strains and antibiotic resistance of the isolates in raw milk and unpasteurized cheese. out of 200 samples of raw milk and 50 samples of unpasteurized cheeses, 96 and 24 strains of e. coli were isolated, respectively. polymerase chain reaction (pcr) was used to detect the genes encodi...

phylogenetic analysis has shown that escherichia coli is composed of four main phylogenetic groups (a, b1, b2 and d). characterization of phylogenetic groups is of clinical interest, as group a and b1 generally associated with commensals, whereas most enteropathogenic isolates are assigned to group d, and group b2 is associated with extra-intestinal pathotype. one hundred e. coli strains recove...

background and objective: to evaluate extended spectrum betalactamase (esbl) positive strains of klebsiella pneumonia and escherichia coli in positive bacterial cultures. materials and methods: in this analytical cross-sectional study, between march 2006 and march 2007, 170 bacterial isolates including 133 cases of e. coli and 37cases of k. pneumonia were examined. all cases underwent double di...

background and objectives: sub-minimum inhibitory concentration (s-mic) can induce changes in morphology, virulence factors and biochemical properties ofbacterial pathogens. the goal of this study is to determine the sub-mic effect of ciprofloxacin and ampicillin on the haemotytic activity of e. coli. material and methods:two clinical isolates of e. coli with high heamolytic activity was select...

One hundred and ten clinical Escherichia coli isolates of serovar O157 (n = 102) and O26 (n = 8) were characterized for the presence of putative virulence genes by PCR. All but one of these isolates contained the eae gene. The EHEC-hly gene could be detected in all E. coli O157 and in 50% of E. coli O26 isolates. Forty-five (40.9%) of the 110 E. coli were positive for both stx(1) and stx(2) gen...

Enterotoxigenic Escherichia coli (ETEC) is a leading cause of childhood diarrhea in low income countries and in travelers to those areas. Inactivated enterotoxins and colonization factors (CFs) are leading vaccine candidates, therefore it is important to determine the prevailing CF types in different geographic locations and populations. Here we developed real time PCR (qPCR) assays for 14 colo...

BACKGROUND AND OBJECTIVE Antibiotic resistance is increasing, especially in healthcare-associated infections causing significant public health concerns worldwide. National information is required to make appropriate policies, update list of essential drugs for treatment, and evaluate the effects of intervention strategies. A nationwide surveillance of antimicrobial resistant bacteria in nosocom...

The aim of this study was to investigate the phylogenetic background and to assess hlyD (involved in the secretion of haemolysin A) and intI1 (encoding a class 1 integrase) in Escherichia coli isolates derived from urinary and fecal specimens. A total of 200 E. coli isolates was collected from patients presenting with urinary tract infection (UTI) during September 2009 to September 2010 and scr...

The study investigated the microbial and sensory quality of powdered beverages produced from blends baobab pulp moringa leave flour. Samples were prepared stored in sterile plastic containers bottles, at room temperature 27±2 0c refrigerator for 21days. Sensory evaluation was done using following parameters, appearance, mouth fill, taste, flavor general acceptability. samples most preferred rel...