University of Wisconsin (UW) solution is not optimal for preservation of marginal organs. Polyethylene glycol (PEG) could improve protection. Similarly formulated solutions containing either 15 or 20 g/L PEG 20 kDa or 5, 15 and 30 g/L PEG 35 kDa were tested in vitro on kidney endothelial cells, ex vivo on preserved kidneys, and in vivo in a pig kidney autograft model. In vitro, all PEGs provide...

We have developed a highly sensitive radioimmunoassay of thyroxine and thyrotropin for mass screening for neonatal hypothyroidism. This assay involves a single disc (3 mm diameter) of dried blood on filter paper. The minimum detectable concentrations are 15 pg/tube (10 microgram/L) for thyroxine and 15 nano-int. units/tube (10 milli-int. units/L) for thyrotropin; intra- and interassay CV's are ...

Two hundred twenty-four steers (initial BW = 363 ± 1.57 kg) were used in a 2 × 3 + 1 factorial arrangement of treatments to evaluate the interactive effects of concentration of wet corn gluten feed (WCGF) and bulk density (BD) of steam-flaked corn (SFC) on feedlot performance, carcass characteristics, and apparent total tract digestibility. Diets consisted of 0, 15, or 30% WCGF (DM basis) with ...

The effects of phytohormone Kinetin on cell density, photosynthetic pigments, antioxidant enzymes, and fatty acids composition the microalgae Tetraselmis suecica were investigated. T. was treated with different concentrations (0, 5, 10, 15, 20, 25 mg/L) at optimal growth conditions (salinity 28 g/L, temperature 25°C, 24 hr photoperiod). Results indicated an increase in density all treatments co...

California Mozambique tilapia (Oreochromis mossambicus x O. urolepis hornorum) are extremely saline tolerant and have been previously shown to reduce whole-animal oxygen consumption rate (MO(2)) upon exposures to salinities greater than that of seawater (SW). In this study tilapia were acclimated to 15, 30, 45, 60 and 75 g/L salinity for 1, 5, 14, or 28 days. There was little change in plasma o...

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conclusions light and temperature were both relevant factors that contributed to the growth and beta-carotene accumulation of the three d. salina strains, and nahco3 had significantly positive effects on growth. the degree of impact of the different factors on cell growth was temperature > nahco3 > light intensity > kno3 > co (nh2)2 > strains; the impact on beta-carotene accumulation was temper...