This paper analyses provable security proofs, using the EDL signature scheme as its case study, and interprets their benefits and drawbacks when applied to the real world. Provable security has been an area of contention. Some, such as Koblitz and Menezes, give little credit to the potential extra security provided and argue that it is a distracting goal. However, others believe that an algorit...

It is often wondered as to what part of all the research performed around the globe actually leaves the lab and reaches the real people and betters their lives. In case of Professor David W. Denning's research, a large part does. As a prominent international face in the field of medical mycology, he translates his research into clinical applications and takes medical problems back to his lab, i...

A gram-negative bacterium, Sphingomonas sp. strain A1, isolated as a producer of alginate lyase, has a characteristic cell envelope structure and forms a mouth-like pit on its surface. The pit is produced only when the cells have to incorporate and assimilate alginate. An alginate uptake-deficient mutant was derived from cells of strain A1. One open reading frame, algS (1,089 bp), exhibiting ho...

the fundamental belief in sikhism is that god exists not merely as an idea or concept, but as a real entity. god is indescribable, yet knowable and perceivable to anyone who is prepared to dedicate the time and energy to become perceptive to his persona. the gurus never spoke about proofs of the existence of god. for them, he is too real, and something obvious does not require any logical proof...

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydr...

The cDNA of the human GM2-activator protein was cloned into the expression vector pHX17. The plasmid encodes a fusion protein with a hexahistidine tail and a Factor Xa cleavage site at its N-terminus. The recombinant protein was purified from cell homogenates under denaturing conditions by metal-ion affinity chromatography in a single step and then was refolded. The hexahistidine tail could be ...