Separation of Trametes versicolor laccase from a commercial preparation was carried out using Three-Phase-Partitioning (TPP) technique. The conditions were optimized and the most favorable ammonium sulphate concentration (70% w/v), crude to t-butanol ratio (1.0:2.0) and pH (4.5) resulted in 56.22% yield with 1.65-fold purity of laccase. For further improvement in purification, an improved versi...

Attempts have been made to produce a subunit influenza vaccine containing haemagglutinin and neuraminidase but free of lipid and nucleic acid. Disintegration of virus with Nonidet P40 followed by selective dialysis was only partially successful, but by disintegration with sodium dodecyl sulphate followed by ether extraction and precipitation with ammonium sulphate a product was obtained in whic...

1. Two soluble arylsulphatases (A and B) have been extracted from ox brain by a modified Albers autolysis method and purified by acetone and ammonium sulphate precipitation and dialysis. 2. A 1600-fold purification was achieved with arylsulphatase A and 320-fold purification with arylsulphatase B. 3. The specific activity of arylsulphatase A was 266000 4-nitrocatechol units/mg. of protein N, an...

Urate oxidase (uricase) was isolated and purified from Pseudomonas aeruginosa to apparent homogeneity using ammonium sulphate precipitation followed by ion exchange and gel filtration chromatography. The specific activity of the purified uricase enzyme was found to be 636.36 with the use of uric acid as a substrate. The purified uricase enzyme is a monomeric protein with molecular weight of 64 ...

1. Pea-seed phosphofructokinase was purified 27-fold by a combination of fractionation with ethanol and ammonium sulphate. Under the conditions of assay, the enzyme was strongly inhibited by phosphoenolpyruvate. This inhibition was reversed by increasing the concentration of fructose 6-phosphate or magnesium chloride, or by lowering the ATP concentration. 2. Citrate, ADP and AMP inhibited phosp...

FROMAGEOT & DESNUELLE [1935] proposed a method of estimation of pyruvic acid based on the oxidation of x-ketonic acids by ceric sulphate. The value of the method is diminished by the fact that numerous substances commonly encountered in biological material, e.g. lactic and citric acids, also react with ceric sulphate. The interference by lactic acid is not serious so long as the amount of the l...

McPhail traps loaded with various attractant solutions (ammonium sulphate, Entomela 75%, Entomela 50%, Dacus bait 100, sexual pheromone of olive fruit fly Bactrocera oleae) combined also with registered plant protection products (lambda-cyhalothrin, alpha-cypermethrin as well as dimethoate) were tested for their attractiveness which was indicated as captures of flies in McPhail traps during thr...

Food colorants from natural sources are preferred over the synthetic variants which elicits various adverse effects including teratogenicity and carcinogenicity. Pigments produced from Monascus spp. are of microbial origin and can be used as food grade biocolorants. The aim of the present work was to investigate the feasibility of Jackfruit seed as a substrate supplemented with carbon sources l...