We describe an assay for in vivo protein interactions. Protein fusions containing ubiquitin, a 76-residue, single-domain protein, are rapidly cleaved in vivo by ubiquitin-specific proteases, which recognize the folded conformation of ubiquitin. When a C-terminal fragment of ubiquitin (C(ub)) is expressed as a fusion to a reporter protein, the fusion is cleaved only if an N-terminal fragment of ...

Background and Objectives: Split sagittal osteotomy is a common operation that may induce inferior alveolar nerve (IAN) damage, potentially leading to sensory deficit, numbness, and pain. Investigations in Iran to evaluate the adverse effects of sagittal split osteotomy surgery are rare so questions have been raised about the success rate of operatio...

BACKGROUND Use of scalp as a donor site was reported for the first time in 1964. Since then, authors have described scalp as a donor site, which heals rapidly and re-growth of hair conceals the donor site. This study was aimed at evaluation of scalp as donor site by calculating the healing time, and frequency of post-operative complications. METHODS This was a descriptive study and was conduc...

the edge tenacity te(g) of a graph g is dened as:te(g) = min {[|x|+τ(g-x)]/[ω(g-x)-1]|x ⊆ e(g) and ω(g-x) > 1} where the minimum is taken over every edge-cutset x that separates g into ω(g - x) components, and by τ(g - x) we denote the order of a largest component of g. the objective of this paper is to determine this quantity for split graphs. let g = (z; i; e) be a noncomplete connected spli...

land surface temperature (lst) is a significant parameter for many applications. many studies have proposedvarious algorithms, such as the split-window method, for retrieving surface temperatures from two spectrallyadjacent thermal infrared bands of satellite data. each algorithm is developed for a limited study area andapplication. in this paper, as part of developing an optimal split-window m...