1. Comparison of the infections of chick embryos by the chorio-allantoic route indicates that Bacterium tularense and Brucella suis, abortus, and melitensis exhibit varying degrees of facultative intracellular parasitism. Pasteurella pestis is adapted to rapid proliferation and spread in the intercellular fluids. 2. In the early stages of infection Bacterium tularense has a marked affinity for ...

A colony blot enzyme-linked immunosorbent assay was developed for the rapid identification of smooth Brucella species, i.e., Brucella abortus, B. melitensis, and B. suis. Bacterial colonies from plates were blotted onto nitrocellulose disks, lysed by immersion in chloroform, and reacted with BRU 38, a rat monoclonal antibody with specificity for the O side chain of B. abortus. Reaction with ant...

A rapid and simple method for the real time monitoring of Brucella abortus CSP-31 (B. abortus CSP-31) by using Surface Plasmon resonance (SPR) is first time reported. SPR and electrochemical impedance spectroscopy (EIS) were used to characterize the B. abortus CSP-31 interaction on carboxymethyldextran modified gold disc with antibody. The EIS data revealed a decrease in impedance after interac...

Korean red ginseng (KRG) has long been used in traditional Korean and Oriental medicine. However, the anti-bacterial mechanism and therapeutic efficiency of KGR for intracellular Brucella infection are still unclear. In this study, the bactericidal activity of Korean red ginseng acidic polysaccharide (RGAP) on Brucella (B.) abortus and its cytotoxic effects on RAW 264.7 cells were evaluated. In...

Of the bacterial antagonists thus far known to be produced by microorganisms, the most effective in bactericidal action against Brucella abortus is the culture filtrate from a strain of Penicillium notatum (Kocholaty, 1942). Another antibacterial substance, penicillin, also obtained from a culture of a strain of Penicillium notatum, but with very little potency against Brucella abortus, has bee...

The chronicity of Brucella abortus infection in humans and animals depends on the organism's ability to escape host defenses by gaining entry and surviving inside the macrophage. Although no human vaccine exists for Brucella, vaccine development in other bacteria has been based on deletions of selective nutritional as well as regulatory systems. Our goal is to develop a vaccine for Brucella. To...

Outer membrane vesicles (OMVs) released by some gram-negative bacteria have been shown to exert immunomodulatory effects that favor the establishment of the infection. The aim of the present study was to assess the interaction of OMVs from Brucella abortus with human epithelial cells (HeLa) and monocytes (THP-1), and the potential immunomodulatory effects they may exert. Using confocal microsco...

Brucellosis is an infection that affects mammals’ cells caused by a species from the genus Brucella, gram-negative, immobile, facultative, intracellular, and microaerophilic coccobacillus. In humans, pathogen are Brucella melitensis, B. abortus, suis canis. We presenting case of 62-year-old man with confirmed brucellosis his consequent cardiovascular, cutaneous, gastrointestinal, hepatic, osteo...

The consumption of milk and unpasteurized dairy products contaminated with Brucella bacteria is one of the most important ways of brucellosis transmission to humans. The principal goal of this study was to determine the prevalence of Brucella abortus (B. abortus) and Brucella melitens (B. melitens)in unpasteurized dairy products consumed in Shiraz province. I...

Baker, Phillip J. (University of Wisconsin, Madison), and J. B. Wilson. Chemical composition and biological properties of the endotoxin of Brucella abortus. J. Bacteriol. 90:895-902. 1965.-The ability of endotoxin to induce hypoferremia in mice was used to measure the specific activity of various endotoxin preparations of Escherichia coli and Brucella abortus and to determine the endotoxin cont...