INTRODUCTION: Certain mineral elements dismetabolism is known to play an important part in the pathogenesis of corneal degenerative and dystrophic diseases, as metalloenzymes participate connective tissues metabolism affecting their properties. Thus, keratoconus tissue depleted iron, copper zinc, what could be underlying cause cornea biomechanical properties impairment. Keratoconus modeling com...

The use of ion exchange beads is proposed as standards for the estimation of various elements in biological tissues. Elements to be detected or quantitated in biological tissues occur in three forms: as free ions or salts, in a crystalline phase, and bound to a matrix. For the latter category a standard system using ChelexlOO -jon exchange beads is proposed. Cations can be attached in a control...

A method was developed to determine whether microorganisms mediate the precipitation of manganese(II) in the marine environment. Radioactive Mn(II) was used as a tracer to measure the precipitation (binding and oxidation) of Mn(II) [i.e., the Mn(II) trapped on 0.2-mum membrane filters] in the presence and absence of biological poisons. A variety of antibiotics, fixatives, and metabolic inhibito...

Trace element determination in seawater is analytically challenging due to the typically very low concentrations of the trace elements and the potential interference of the salt matrix. A common way to address the challenge is to pre-concentrate the trace elements on a chelating resin, then rinse the matrix elements from the resin and subsequently elute and detect the trace elements using induc...

The objective of this study was to investigate the risk of attenuated efficacy due to adaptive resistance for the siderophore-conjugated monocarbam SMC-3176 in Pseudomonas aeruginosa by using a pharmacokinetic/pharmacodynamic (PK/PD) approach. MICs were determined in cation-adjusted Mueller-Hinton broth (MHB) and in Chelex-treated, dialyzed MHB (CDMHB). Spontaneous resistance was assessed at 2×...

Acinetobacter baumannii is an opportunistic Gram-negative pathogen that causes a wide range of infections including pneumonia, septicemia, necrotizing fasciitis and severe wound and urinary tract infections. Analysis of A. baumannii representative strains grown in Chelex 100-treated medium for hemolytic activity demonstrated that this pathogen is increasingly hemolytic to sheep, human and horse...

PURPOSE To determine the relative rate of oxidation of ascorbic acid (ASA) and glucose under conditions used for glycation reactions in vitro and by ultraviolet A (UVA)-generated oxygen free radicals using human lens sensitizers. METHODS ASA and [14C]glucose were incubated in 0.1 M phosphate buffer, and the rate of oxidation was determined by absorbance at 265 nm and by thin-layer chromatogra...

The aim of this study was to determine the genetic diversity of Plasmodium falciparum by analyzing the polymorphism of the msp-1 and msp-2 genes and the multiplicity of infection in children with uncomplicated malaria in southern Benin. Blood samples of children with fever or history of fever with thick smear positive P. falciparum were collected on filter paper. After extraction of DNA by Chel...