نتایج جستجو برای: clamp techniques

تعداد نتایج: 648187  

Journal: :American journal of physiology. Endocrinology and metabolism 2000
Z Q Sun K Ojamaa W A Coetzee M Artman I Klein

Thyroid hormones play an important role in cardiac electrophysiology through both genomic and nongenomic mechanisms of action. The effects of triiodothyronine (T(3)) on the electrophysiological properties of ventricular myocytes isolated from euthyroid and hypothyroid rats were studied using whole cell patch clamp techniques. Hypothyroid ventricular myocytes showed significantly prolonged actio...

Journal: :Biophysical journal 1999
A J Sherman A Shrier E Cooper

Whole-cell patch-clamp techniques are widely used to measure membrane currents from isolated cells. While suitable for a broad range of ionic currents, the series resistance (R(s)) of the recording pipette limits the bandwidth of the whole-cell configuration, making it difficult to measure rapid ionic currents. To increase bandwidth, it is necessary to compensate for R(s). Most methods of R(s) ...

Journal: :Journal of neurophysiology 1997
Z L Mo R L Davis

Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from postnatal mouse spiral ganglion neurons in vitro. Cultures contained neurons that displayed monopolar, bipolar, and pseudomonopolar morphologies. Additionally, a class of neurons having exceptionally large somata was observed. Frequency histograms of the maximum number of action pot...

Journal: :American journal of physiology. Heart and circulatory physiology 2000
J T Hulme C H Orchard

The effect of acidosis on the transient outward K(+) current (I(to)) of rat ventricular myocytes has been investigated using the perforated patch-clamp technique. When the holding potential was -80 mV, depolarizing pulses to potentials positive to -20 mV activated I(to) in subepicardial cells but activated little I(to) in subendocardial cells. Exposure to an acid solution (pH 6.5) had no signif...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2014
Navid Bavi Yoshitaka Nakayama Omid Bavi Charles D Cox Qing-Hua Qin Boris Martinac

The lipid bilayer plays a crucial role in gating of mechanosensitive (MS) channels. Hence it is imperative to elucidate the rheological properties of lipid membranes. Herein we introduce a framework to characterize the mechanical properties of lipid bilayers by combining micropipette aspiration (MA) with theoretical modeling. Our results reveal that excised liposome patch fluorometry is superio...

Journal: :The Journal of pharmacology and experimental therapeutics 2002
Maddison D Harrell Joseph R Stimers

9,10-Epoxy-12-octadecenoic acid (EOA), a metabolite of linoleic acid, causes cardiac arrest in dogs. Other metabolites of linoleic acid also have toxic effects. This study investigates the mechanism of action of four of these compounds on cardiac Na(+) current (I(Na)). The whole-cell patch-clamp technique was used to investigate the effects of EOA, 9,10-dihydroxy-12-octadecenoic acid (DHOA), an...

Journal: :Journal of neurophysiology 2002
Weihong Lin Tatsuya Ogura Sue C Kinnamon

Sour taste is mediated by acids with the degree of sourness a function of proton concentration. Recently, several members of the acid-sensing ion channel subfamily (ASICs) were cloned from taste cells and proposed to mediate sour taste. However, it is not known whether sour responses in taste cells resemble the responses mediated by ASICs. Using the whole cell patch-clamp technique and Na(+) im...

Journal: :The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2011
Pawan Kumar Shahi Seok Choi Dong Chuan Zuo Cheol Ho Yeum Pyung Jin Yoon Jun Lee Young Dae Kim Chan Guk Park Man Yoo Kim Hye Rang Shin Hyun Jung Oh Jae Yeoul Jun

In this study we determined whether or not 5-hydroxytryptamine (5-HT) has an effect on the pacemaker activities of interstitial cells of Cajal (ICC) from the mouse small intestine. The actions of 5-HT on pacemaker activities were investigated using a whole-cell patch-clamp technique, intracellular Ca(2+) ([Ca(2+)](i)) analysis, and RT-PCR in ICC. Exogenously-treated 5-HT showed tonic inward cur...

Journal: :Analytical chemistry 2008
Kai F Hoettges Yvonne Hübner Lionel M Broche Stephen L Ogin George E N Kass Michael P Hughes

Dielectrophoresis (DEP) offers many advantages over conventional cell assays such as flow cytometry and patch clamp techniques for assessing cell electrophysiology as a marker for cancer studies and drug interaction assessment. However, despite the advantages offered by DEP analysis, uptake has been low, remaining largely in the academic arena, due to the process of analysis being time-consumin...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 1996
F M Zhou J J Hablitz

Using whole-cell patch-clamp techniques in brain slices, we studied the postnatal development of electrophysiological properties of rat neocortical layer I neurons during the first three weeks of postnatal life. Neurons, including Cajal-Retzius cells, were visualized under Nomarski optics before recording. In the first postnatal week, all layer I neurons, including Cajal-Retzius cells, had low ...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید