DNA barcoding is a simple method for the identification of any species using a short genetic sequence from a standard genome section. The present study aimed at examining the nuclear and chloroplast diversity as well as the phylogenetic relationships of eight species of saffron including four spring-flowering and five autumn-flowering species from different parts of Iran, using the nuclear barc...

A reference collection of COI barcode (650 bp) for the Pacific Society Islands has been constituted for 22 species of Acanthuridae and 16 species of Holocentridae. Divergence between congeneric species was on average 20-fold to 87-fold higher than divergence between conspecific sequences and this set of DNA-identifiers was used to identify 40 larvae of both families. All larvae sequenced could ...

Abstract We establish a new genus of planocerid polyclad, Heteroplanocera gen. nov., based on species, H. katoi sp. collected at depth 245 m off the coast Izu Peninsula, West Pacific, Japan. nov. is distinguished from other genera by following characteristics: (i) pair nuchal tentacles, (ii) muscular bulb, (iii) spermiducal bulbs (instead seminal vesicle), (iv) free prostatic vesicle, (v) acces...

The COI gene is widely used as a DNA barcode in animals that can assist the identification of species. One aggregators barcodes Barcode Life Data System (BOLD Systems), which contains around 2500 sequences Tetrigidae, an understudied orthopteran family with unresolved taxonomy and species are difficult to identify. In this paper, we provide summary metadata provided present phylogenetic analysi...

Mosquitoes are a diverse group of invertebrates, with members that are among the most important vectors of diseases. The correct identification of mosquitoes is paramount to the control of the diseases that they transmit. However, morphological techniques depend on the quality of the specimen and often unavailable taxonomic expertise, which may still not be able to distinguish mosquitoes among ...

PrimerMiner: an R package for development and in silico validation of DNA 1 metabarcoding primers 2 3 Vasco Elbrecht *, Florian Leese 1,2 4 5 Affiliations: 6 1) Aquatic Ecosystem Research, Faculty of Biology, University of Duisburg-Essen, Universitätsstraße 5, 45141 Essen, 7 Germany 8 2) Centre for Water and Environmental Research (ZWU) Essen, University of Duisburg-Essen, Universitätsstraße 2,...

Examining species boundaries using data from multiple independent sources is an appropriate and robust method to identify genetically isolated evolutionary units. We used 5 data sets—cytochrome b (Cytb), cytochrome c oxidase (COI), amplified fragment length polymorphisms (AFLPs), karyotypes, and morphology—to estimate phylogenetic relationships and species limits within woolly bats, genus Keriv...

BACKGROUND DNA barcoding is one means of establishing a rapid, accurate, and cost-effective system for the identification of species. It involves the use of short, standard gene targets to create sequence profiles of known species against sequences of unknowns that can be matched and subsequently identified. The Fish Barcode of Life (FISH-BOL) campaign has the primary goal of gathering DNA barc...

Mosquitoes are a diverse group of invertebrates, with members that are among the most important vectors of diseases. The correct identification of mosquitoes is paramount to the control of the diseases that they transmit. However, morphological techniques depend on the quality of the specimen and often unavailable taxonomic expertise, which may still not be able to distinguish mosquitoes among ...

The Ostracoda (Crustacea; Class Ostracoda) is a diverse, frequently abundant, and ecologically important component of the marine zooplankton assemblage. There are more than 200 described species of marine planktonic ostracods, many of which (especially conspecific species) can be identified only by microscopic examination and dissection of fragile morphological characters. Given the complexity ...