OBJECTIVE To report the outcome of mushroom keratoplasty for the treatment of full thickness corneal disease in pediatric patients with healthy endothelium. METHODS A retrospective analysis of pediatric patients who underwent mushroom keratoplasty. The medical records of pediatric patients suffering from full thickness corneal stromal disease with normal endothelium who underwent mushroom ker...

Full-thickness explants of rabbit cornea were grown in organ culture and the migratory behavior of both epithelium and endothelium was observed under different experimental conditions. Following explantation, epithelial migration along the cut surface of the stroma began within six hours. By 24 hours, the epithelium reached the cut edge of Descemet's membrane and made contact with the endotheli...

To determine the mechanism of latrunculin B (LAT-B)-induced decrease in outflow resistance and the effect of LAT-B on the cornea, structural changes of the trabecular meshwork (TM) and the corneal endothelium following LAT-B were studied in the live monkey eye. LAT-B (0.5 microM) and vehicle were administered by anterior chamber exchange and infusion with cationized and non-cationized gold solu...

Background: Elevated intraocular pressure (IOP) is one of the most important factors for diagnosis and monitoring glaucoma. IOP currently only modifiable risk factor glaucoma, cornea transparent tissue covering front eye. It a powerful refracting surface, providing 2/3 eye's focusing power. The adult about 1/2 millimeter thick comprised 6 layers: epithelium, Bowman's membrane, stroma, Dua's lay...

Purpose: To describe a rare bilateral corneal ringshaped opacity, not categorized as corneal dystrophy or degeneration occurring without any associated ocular or systemic disease. Methods: Case report and review of the literature. Results: The authors describe the clinical history and examination of an 80 years old man, with an asymptomatic bilateral and symmetrical ring-shaped corneal opacity ...

Rabbit peritoneal polymorphonuclear leukocyte lysosomes were injected into the anterior chamber. The cornea was studied in paraffin cross-sections. To study the corneal endothelium, fiat preparations were made. Nitroblue tetrazolium staining was used to study endothelial cell viability. The mitotic activity of the endothelium was estimated by tritiated thymidine incorporation. The corneas of ly...

A water permeability study of the isolated cat corneal endothelium was performed with the use of a newly designed Lucite chamber and modified experimental methods. The hydraulic conductivity of the cat endothelium was 9.3 ± 1.09 x 10~ cm. seer atmr. The Staverman reflection coefficient to NaCl of cat endothelium was 0.6. It was determined that the resistance to the passage of either water or sm...

Complement is present in ocular fluids, but the molecular mechanism(s) restricting its activation to exogenous targets and not to autologous ocular cells are currently unknown. To clarify how this control is achieved, monoclonal antibody (mAb)-based techniques were used to examine the eye, the lacrimal gland, and ocular fluids for the decay-accelerating factor (DAF), a membrane regulatory prote...

Physiological studies have demonstrated that ions, as well as large molecules such as hemoglobin or fluorescein, can diffuse across and within the cornea. Most of the substrates for corneal metabolism are obtained from aqueous humor filling the anterior chamber. In order to receive its nutrients and in order to maintain its normal conditions of hydration, the avascular cornea must transport rel...