To investigate the effects of new two low-shrinkage composites SDR(®) and Venus(®)Bulk Fill on the cell viability, cellular damage and expression of mesenchymal markers on dental stem cells. Specimens from two low-shrinkage composites were eluted with culture medium for 24 h. After 24 h of incubation, cytotoxicity of elutes were evaluated by MTT assay; apoptosis was determined using the DNA-spe...

Objective. To analyze the latest literature data on possibilities of using stem cells odontogenic origin in practice regenerative medicine.Material and methods. The bibliosemantic method was applied, structural-logical analysis received carried out. Electronic databases such as PubMed, MEDLINE, Scopus, Web Science EMBASE were used for modern scientific searching by keywords «regenerative medici...

WNT signaling pathway functions throughout the lifetime of the organism by ensuring the normal patterning of tissues during development and by regulating selfrenewal and cell fate commitment in multiple adult stem cell types. Currently, not enough is known about the role of WNT signaling in the maintenance of adult dental tissues. In this review we briefly outline current developments in the fi...

The success of bioengineered dental pulp depends on two principles, (1) whether the transplanted tissue can develop its own vascular endothelial tubule network and (2) whether the host vasculature can be induced to penetrate the bioengineered pulp replacement and conjoin. Major inductive molecules that participate in laying down blood vessels include vascular endothelial growth factor (VEGF), e...

Introduction: Although missing tooth is not life-threatening, it affects the quality of daily life. Stem cells have emerged as an important player in generation and maintenance many tissues. The role scaffolds has changed from a passive carrier to bioactive matrix, which can be used induce cellular behavior. aim this study was determine possibility regeneration dentin-pulp complex with dental p...

This systematic review evaluated if different cryopreservation protocols could affect biological properties (Cell survival rate (CSR), proliferation, differentiation, maintenance of stem cell markers) of stem cells obtained from dental tissues (DSC) post-thaw. An electronic search was carried out within PubMed and ISI Web Science by using specific keyword. Two independent reviewers read the tit...

The pluripotent nature of embryonic stem cells (ESCs) makes them amenable for regenerative therapies because they can differentiate into cells that form all tissue types within the body (Zandstra and Nagy, 2001). The potential drawbacks to the use of ESCs for cellular therapies include the obvious ethical dilemmas of obtaining ESCs, the potential of cancer or tumor formation and the risk of imm...

Objective(s): Osteonecrosis of the jaw, as an exposed necrotic bone in the oral cavity, is one of the adverse effects of bisphosphonates, which have an affinity for bone minerals. This study investigates the cytotoxic effects of alendronate (ALN) as a nitrogen-containing bisphosphonate, on human dental pulp stem cells (hDPSCs). Materials and Methods: The mesenchymal stem cells (MSCs), obtained ...

Mesenchymal stem cells (MSCs) are adult multipotent stem cells that are capable of self-renewal and differentiation into multiple cell lineages. Methods for cell therapy using MSCs have been developed in equine medicine. Recently, human dental pulp stem cells (DPSCs) have drawn much attention owing to their trophic factor producing ability and minimally invasive collection methods. However, the...

Dental pulp is a highly specialized mesenchymal tissue that has a limited regeneration capacity due to anatomical arrangement and post-mitotic nature of odontoblastic cells. Entire pulp amputation followed by pulp space disinfection and filling with an artificial material cause loss of a significant amount of dentin leaving as life-lasting sequelae a non-vital and weakened tooth. However, regen...