Replication of DNA is carried out by the replisome, a multiprotein complex responsible for the unwinding of parental DNA and the synthesis of DNA on each of the two DNA strands. The impressive speed and processivity with which the replisome duplicates DNA are a result of a set of tightly regulated interactions between the replication proteins. The transient nature of these protein interactions ...

Data Grid is an infrastructure that manages huge amount of data files, and provides intensive computational resources across geographically distributed collaboration. To increase resource availability and to ease resource sharing in such environment, there is a need for replication services. Data replication is one of the methods used to improve the performance of data access in distributed sys...

The physical organization of DNA enzymes at a replication fork enables efficient copying of two antiparallel DNA strands, yet dynamic protein interactions within the replication complex complicate replisome structural studies. We employed a combination of crystallographic, native mass spectrometry and small-angle X-ray scattering experiments to capture alternative structures of a model replicat...

In the interest of achieving higher reliability, mobile ad hoc grid should apply a fault tolerance in resource allocation scheme. Resource allocation plays a critical role in mobile ad hoc grids due to the dynamic nature of nodes connection. Allocating appropriate resources for the tasks can avoid uncompleted tasks and ensure service reliability. We propose two approaches for avoiding tasks fai...

Replisomes were originally thought to be multi-protein machines with a stabile and defined structure during replication. Discovery that replisomes repeatedly discard sliding clamps and assemble a new clamp to start each Okazaki fragment provided the first hint that the replisome structure changes during replication. Recent studies reveal that the replisome is more dynamic than ever thought poss...

The evolving of vehicular network applications, from simple alert message exchanging to more elaborated and sophisticated systems, boosts the need for content delivery solutions. A useful technique, in such case, is the content replication, in which strategically selected vehicles replicate content and help on the delivery process. However, content replication is particularly challenging in veh...

Replication can enhance the availability of the data les in a distributed environment. This paper introduces a method for managing replicated data les. Unlike many others, our method provides protocols and algorithms for a more complicated scheme of replication that supports replication with location-variant les and les with a variable degree of replication. We assume the existence of a dynamic...

DNA replication, similar to other cellular processes, occurs within dynamic macromolecular structures. Any comprehensive understanding ultimately requires quantitative data to establish and test models of genome duplication. We used two different super-resolution light microscopy techniques to directly measure and compare the size and numbers of replication foci in mammalian cells. This analysi...

Postreplicative maintenance of genomic methylation patterns was proposed to depend largely on the binding of DNA methyltransferase 1 (Dnmt1) to PCNA, a core component of the replication machinery. We investigated how the slow and discontinuous DNA methylation could be mechanistically linked with fast and processive DNA replication. Using photobleaching and quantitative live cell imaging we show...