نتایج جستجو برای: embryonic ciliation
تعداد نتایج: 80499 فیلتر نتایج به سال:
Introduction: Type I diabetes mellitus is caused by autoimmune destruction of the insulin-producing β-cells. A new potential method for curing the disease is transplantation of differentiated insulin- secreting cells from human embryonic stem cells. Methods: Human embryonic stem cell lines (Royan H1) were used to produce embryoid bodies. Differentiation carried out by growth factor-mediated se...
Background: Primary clear cell adenocarcinoma of cervix (CCAC) is usually seen in women with a history of in utero exposure to diethyl acetyl bestrol (DES). We report two cases of clear cell adenocarcinoma of cervix with no history of exposure to DES in embryonic period. Case presentation: The first case was a 14-year-old women with complaint of painless vaginal bleeding. There was atypical ce...
Backgrounds: A straight-forward way to identify whether a drug or environmental chemical can be harmful to unborn baby is to evaluate its effect on laboratory animals. All invivo methods need large number of animal and are therefore time consuming and expensive. However, the thousands of chemicals in need of testing, to reduce the spending of live animals, an assortment of in vitro assays has ...
Background: In mammalian ovaries, loss of over two-thirds of germ cells happens due to cell death. Nonetheless, the exact mechanism of cell death has yet to be determined. The present basic practical study was designed to detect 3 types of programmed cell death, namely apoptosis, autophagy, and necrosis, in murine embryonic gonadal ridges and neonatal ovaries.Methods: Twenty gonadal ridges and ...
Purpose: Differentiation of mouse embryonic stem cells into Insulin secreting endocrine cells. Materials and Methods: In this study, Royan B1 mouse embryonic stem cell (derived from C57BL/6 mouse) were used. In directed differentiation method, embryonicstem cells after embryoid bodies formation were differentiated into insulin secreting cells. Nestin positive cells were obtained after culture ...
An experiment was conducted to assess how hatchability performance of eggs is affected by line and age female broiler breeders. Response variables analyzed were hatchability, infertility, pipping (i.e., pipped shell but not emerged), embryonic mortality (1st, 2nd, 3rd wk), malposition. The trials involved a total 2,880 fertile from two breeder lines (Ross 308 Cobb 500) at different ages (30 50 ...
background: this study was conducted to evaluate fibroblast co-culture and activin a on in vitro maturation and fertilization of mouse preantral follicles. methods: the ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-mem as based medium for 12 days. a total number of 456 follicles were cultured in four conditions: (i) base mediu...
introduction: different effects of low level laser irradiation (llli) on various cell types have already been demonstrated. however, its effects on embryonic stem cells have not yet been shown. the present study evaluates the morphological and immunocytochemical effects of llli on human embryonic stem cell (hesc) colonies. material and methods: equal-sized pieces of hesc line (royan h1) were ir...
Recently new reports have proved the pluripotency of spermatogonial stem cells (SSCs) derived from male gonad. This pluripotent stem cells resembled Embryonic stem cells recognized as Embryonic Stem like cells (ES like cells). ES like cells forms sharp edge colonies that are immunopositive to pluripotency markers and have differentiation capacity to Ectodermal, Mesodermal and Endodermal layers....
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