Title of Dissertation: IMMOBILIZATION OF ENZYMES ON NANOPOROUS SILICA COMPOSITES Allan E. David, Ph.D., 2004 Dissertation Directed By: Associate Professor N.S. Wang, Department of Chemical Engineering Dr. A.J. Yang, ISTN, Inc. In this work, the sol-gel process is used to produce a chemically surface modified gel (CSMG) with a high modification density. Sodium silicate, an inexpensive alternativ...

Studies were conducted on the Bacillus sp. neutral protease immobilization on different matrices. The immobilized protease was prepared by three methods, such as entrapment on calcium alginate and gelatin; physical adsorption by celite and alumina; ionic binding by amberlite IR-120 and DEAE-cellulose. The maximum immobilization yield was obtained with celite adsorbed protease. The immobilized e...

This paper reports on a novel and efficient β-glucosidase immobilization method using magnetic Fe3O4 nanoparticles as a carrier. Based on response surface methodology, the optimal immobilization conditions obtained were: glutaraldehyde (GA) concentration, 0.20%; enzyme concentration, 50.25 μg/mL; cross-linking time, 2.21 h; and the maximum activity recovery reached 89.35%. The magnetic immobili...

Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion of glutamic acid to gamma-aminobutyric acid (GABA), was fused to the cellulose-binding domain (CBD) and a linker of Trichoderma harzianum endoglucanase II. To prevent proteolysis of the fusion protein, the native linker was replaced with a S(3)N(10) peptide known to be completely resistant to E. coli...

Bifunctional fusion proteins consisting of organophosphate hydrolase (OPH) moieties linked to a Clostridium-derived cellulose-binding domain (CBD) were shown to be highly effective in degrading organophosphate nerve agents, enabling purification and immobilization onto different cellulose materials in essentially a single step. Enzyme kinetics studies were performed for the CBD-OPH fusions usin...

Abstract This study evaluated the lactose hydrolysis by immobilized ?-galactosidase, which was produced Kluyveromyces marxianus using porungo cheese whey as substrate. Initially, yeast cultivated in medium at 30 °C and 200 rpm, showing a maximal ?-galactosidase production of 14.19 U mL-1. The crude extract obtained used to evaluate enzymatic solution. optimal pH temperature free enzyme investig...

Owing to various industrial applications of mercury (Hg), its release into the environment at high concentration is becoming a great threat living organisms on global scale. Human exposure Hg greatly correlated with contamination in food chain through cereal crops and sea foods. Since non-essential component does not possess biological role exhibits carcinogenic genotoxic behaviour, biomonitori...

Enzymes immobilized on synthetic polyethylene terephthalate (PET) textile surface by resource-efficient inkjet printing technology can promote developments for various novel applications. Synthetic fabrics often require adequate pretreatments to facilitate such process. This work discusses PET–woven fabric pretreatment routes improve wettability alkaline, enzymatic, and plasma processes effecti...

Over the past years, DNA biosensors have been developed to analyze DNA interaction and damage that have important applications in biotechnological researches. The immobilization of DNA onto a substrate is one key step for construction of DNA electrochemical biosensors. In this report, a direct approach has been described for immobilization of single strand DNA onto carboxylic acid-functionalize...