A decrease in proteoglycan (Pg) content and disturbances in the collagen network have been reported in aging cartilage. This study aims to determine whether these changes are associated with proteolytic enzymes such as neutral metalloproteases. Eighty lateral tibial plateaus were collected from subjects after death. The age, topographical area, and lesion severity (macroscopic grading) of each ...

background: we aimed at evaluating the toxicity effects of low (subtoxic) concentrations of silver nanoparticles (agnp, 5-10 nm) in human hepatoblastoma (hepg2) cell line after and during a period of about one month. methods: xtt and mtt assays were used to draw a dose-response curve ic50 (half maximal inhibitory concentration) value of the agnp on hepg2 cells was calculated to be 2.75-3.0 mg/l...

In this study, complete purification, characterization, and immobilization of polygalacturonase (pectinase) from Penicillium notatum were carried out to achieve an economical suitable alternative for industrial fruit juice clarification. Biosynthesis was under pre-optimized conditions employing solid-state fermentation at a pilot scale using wheat bran. The enzyme subjected series steps purific...

pioglitazone (pg) is one of thiazolidinediones used for the treatment of type ii diabetes mellitus. some reports of its hepatotoxicity exist, but the mechanism of its hepatotoxicity is not well known. in the present study, the protective effect of some atp suppliers are investigated against mitochondrial toxicity of pg in isolated rat mitochondria. mitochondrial viability was investigated by mt...

In order to study a short-term and efficient technology by 1-methylcyclopropene (1-MCP) in blueberry, the fruit was treated with 0, 0.5, 1 3 μL/L 1-MCP for 2 h then stored at 25 ± °C 40–50% relative humidity (RH) 9 d. The weight loss, decay incidence, respiration rate, firmness, soluble solid content (SSC), titratable acid (TA), Brix-acid ratio (BAR), sensory evaluation, of cell wall polysaccha...

Softening, which accompanies ripening fruits is also to a large extent, responsible for the damage that occurs during handling and shipping. The effects of 1-Methylcyclopropene (1-MCP) application at different ripening stages (mature green, breaker, breaker + 2 days and breaker + 4 days) on cellulase and pectinase activities of cherry tomato fruit (Lycopersicon esculentum Mill. var. cerasiforme...

Ripening of fruit is a very important process but in some fruits early ripening leads to a great damage during long distance transportation. There are various biochemical changes taking place during the phase of ripening of fruit such as changes in respiration, aroma, flavor, ethylene production and activity of cell wall degrading enzymes. Some important cell wall degrading enzymes are Polygala...

Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three transcripts are specifically expressed in salivary glands indicating that PGs are salivary enzymes. T...