We formerly released the porcine expressed sequence tag (EST) database Pig EST Data Explorer (PEDE; http://pede.dna.affrc.go.jp/), which comprised 68,076 high-quality ESTs obtained by using full-length-enriched cDNA libraries derived from seven tissues. We have added eight tissues and cell types to the EST analysis and have integrated 94,555 additional high-quality ESTs into the database. We al...

Figure A Overview of Steps Involved in a Cot Analysis. (A) Nuclear DNA is sheared into 200-500 bp fragments by high-speed blending, and (B) fragment size is checked by gel electrophoresis. (C) Sheared DNA is precipitated, dissolved in 0.03, 0.12, or 0.5 M sodium phosphate buffer (SPB), and aliquots are loaded/sealed into glass microcapillary tubes/ampoules. (D) One tube/ampoule is (E) placed in...

There is considerable interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) to enable the analysis of the potential relationships between human genotype and phenotype. Here we present a strategy that permits the rapid discovery of SNPs from publicly available expressed sequence tag (EST) databases. From a set of ESTs derived from 19 different cDNA libraries, w...

l'objectif de ce travail consiste à étudier le caractère pluriel de l’interprétation du texte littéraire.  ce caractère pluriel se manifeste aussi dans le travail de traduction littéraire qui est elle-même une interprétation. la preuve est la manifestation ou la publication de plusieurs traductions d'un texte littéraire donné dans une culture donnée. ce phénomène n'est pas spécif...

depuis les années 1990, une nouvelle visée critique est venue enrichir la liste déjà riche des perspectives de recherches appliquées à la littérature. aux investigations de type historique, textuelle, sociologique, etc. est venue s’ajouter une tendance critique aux contours encore indéfinis, mais dont la volonté première est de valoriser le rapport parfois apparent, souvent implicite, entre la ...

The genus Cercospora is one of the largest and most heterogeneous genera of hyphomycetes. Cercospora species are distributed worldwide and cause Cercospora leaf spot on most of the major plant families. Numerous species described from diverse hosts and locations are morphologically indistinguishable from C. apii and subsequently are referred to as C. apii sensu lato. The importance and ecologic...

We identified in the EST database murine and human sequences similar, but not identical, to the members of the PC3/BTG/TOB family of cell cycle inhibitors. A conserved domain (aa 50-68) of the PC3 protein, the prototype member of the family, was used as a query. That domain has been shown by us to be necessary for the antiproliferative activity of PC3. A murine EST clone and a highly homologous...