نتایج جستجو برای: fecal indicator bacteria
تعداد نتایج: 275955 فیلتر نتایج به سال:
The US Environmental Protection Agency has proposed the use of quantitative polymerase chain reaction (qPCR) as a rapid alternative analytical method for monitoring recreational water quality at beaches. For qPCR to be considered for other Clean Water Act purposes, such as inclusion in discharge permits and use in Total Maximum Daily Load calculations, it is necessary to understand how qPCR det...
The present study reports on the fecal contamination in Mhlathuze river catchment and the incidence of antibiotic resistance amongst the bacterial isolates from water sources as well as from diarrhoea patients living in the area. The Mhlathuze river catchment (Northern KwaZulu-Natal, RSA) is home to an extensive rural population that is dependent on water from this river for all domestic purpos...
The original use of “indicator” organisms to signal the possible presence of pathogenic bacteria dates back more than a hundred years. Since then, the use of these organisms as predictors of pathogens has been extended from water to a variety of raw and processed food products. When used to predict the possible presence of pathogens, the term “index organism” is now preferred; “indicator organi...
The application of quantitative real-time PCR (qPCR) technologies for the rapid identification of fecal bacteria in environmental waters is being considered for use as a national water quality metric in the United States. The transition from research tool to a standardized protocol requires information on the reproducibility and sources of variation associated with qPCR methodology across labor...
On-site wastewater treatment systems (OWS) are a common means of wastewater treatment in coastal North Carolina, where the soils are sandy and groundwater is relatively close to the surface (<5 m). Wastewater contains elevated concentrations of pathogenic microorganisms that can contaminate groundwater and surface water if OWS are not operating efficiently and distributing wastewater equally to...
Algae on freshwater beaches can serve as reservoirs for fecal indicator bacteria (FIB). Wrack (especially kelp) at marine beaches might sustain FIB as well. This study examines the relationship between beach wrack, FIB, and surrounding water and sediment at marine beaches along the California coast. Surveys of southern and central California beaches were conducted to observe environmental wrack...
Fecal indicator bacteria (FIB) indicative of fecal contamination in urban streams and recreational ocean beaches in Santa Barbara, California often exceed recreational water-quality standards. During low flow, FIB and human-specific Bacteroides concentrations in urban streams were associated with point discharges. FIB concentrations varied three-fold during diurnal sampling as a result of small...
This study examines the use of fecal indicator bacteria (FIB) as a predictor of the presence of Helicobacter spp. A combination of standard culture and molecular techniques were used to detect and quantify FIB, Helicobacter spp. and H. pylori from five North American rivers of different size and with different land use characteristics. Primers designed to amplify genes specific to Helicobacter ...
BACKGROUND The United States Environmental Protection Agency has established methods for testing beach water using the rapid quantitative polymerase chain reaction (qPCR) method, as well as "beach action values" so that the results of such testing can be used to make same-day beach management decisions. Despite its numerous advantages over culture-based monitoring approaches, qPCR monitoring ha...
Continuous monitoring of particle-size distribution (PSD), total organic carbon (TOC), turbidity, discharge and physicochemical parameters, together with analyses of fecal indicator bacteria, particularly Escherichia coli, made it possible to better understand the processes governing pathogen transport in karst groundwater and to establish PSD as indicator for possible microbial contamination o...
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