AIM To develop a high-throughput multiplex, fast and simple assay to scan azoospermia factor (AZF) region microdeletions on the Y chromosome and establish the prevalence of Y chromosomal microdeletions in Chinese infertile males with azoospermia or oligozoospermia. METHODS In total, 178 infertile patients with azoospermia (non-obstructed), 134 infertile patients with oligozoospermia as well a...

Objective To compare the prevalence of sexually transmitted infections (STIs) (including HIV) and of highrisk sexual behaviour in the following three groups: primary infertile relationships, secondary infertile relationships and fertile relationships. Primary infertility is here defined as never having conceived before, secondary infertility as infertility subsequent to having conceived at leas...

This review explores the relationship between sperm chromosomal constitution and morphology. With the advent of techniques for obtaining information on the chromosome complements of spermatozoa, this relationship has been studied in fertile men and in men with a high frequency of chromosomal abnormalities. Using human sperm karyotype analysis, no relationship between sperm chromosome abnormalit...

Materials and methods A total of 500 infertile male patients attending private infertility clinics of Guwahati, Assam, were selected for the study. Blood and semen samples were collected from the patients. Genomic DNA was isolated from both type of samples and PCR amplification was carried out using specific primer sets. The genes and sequence-tagged site (STS) markers included in the study are...

STUDY QUESTION Can a standardized assessment of abnormal human sperm morphology provide additional useful information by identifying men with more severe disturbances in different types of abnormalities? SUMMARY ANSWER Definition-based categorization of sperm head, midpiece and tail defects has shown how differently these abnormalities are distributed in fertile men and other groups of men, t...

The human Y chromosome harbors genes that are responsible for testis development and also for initiation and maintenance of spermatogenesis in adulthood. The long arm of the Y chromosome (Yq) contains many ampliconic and palindromic sequences making it predisposed to self-recombination during spermatogenesis and hence susceptible to intra-chromosomal deletions. Such deletions lead to copy numbe...

Objective: To investigate the association between malondialdehyde concentration in seminal plasma of infertile men and sperm quality.Methods: This case-control study included 60 male participants ranging from 25-40 years old with half them were fertile other infertile. Semen analysis was performed as per WHO standards, spectrophotometric measurement level done.Results: Results showed that had s...