نتایج جستجو برای: in silico cloning
تعداد نتایج: 16991398 فیلتر نتایج به سال:
مقدمه: بروز نتایج متفاوت در آزمایشگاهها به علت استاندارد نبودن، از معایب روشهای تکثیر مولکولی است. در بیشتر مقالات چاپ شده در زمینه تشخیص pcr عوامل عفونی، اینترنال کنترل تکثیری (ic) وجود ندارد. هدف از پژوهش حاضر، طراحی و توسعه یک روش ساده و سریع، برای ساخت اینترنال کنترل آزمون pcr در آزمایشگاههای تشخیصی است. مواد و روشها: در پژوهش حاضر، برای ساخت اینترنال کنترل رقابتی، از پ...
Risk assessment of a transgene is one of the key steps in genetic transformation. Hence, in order to use cryIAc gene for production of transgenic plants, a library and in-silico research was performed to confirm safety of the gene for human comsumption, animal feed and environment. In the first step, the molecular mechanism of action of the CryIAc protein and its specific receptors in the midgu...
In July 2022, a new virus called Langya (LayV) was discovered in China patients who had fever. This is type of Henipavirus (HNV) and considered potential threat as it could spread from animals to humans. It causes respiratory disease with symptoms including fever, coughing, fatigue closely linked two other henipaviruses that are known infect humans, namely Hendra Nipah viruses. These viruses ma...
The properties relevant to pharmacokinetics and pharmacodynamics of four series of synthesized s-triazine derivatives have been studied by Quantitative structure-retention relationship (QSRR) approach. The chromatographic behavior of these compounds was investigated by using reversed-phase high performance thin-layer chromatography (RP-HPTLC). Chromatographic retention (RM0) was correlated with...
identification of a de novo 3bp deletion in cryba1/a3 gene in autosomal dominant congenital cataract
autosomal dominant congenital cataract (adcc) is the most common form of inherited cataracts and accounts for one-third of congenital cataracts. heterozygous null mutations in the crystallin genes are the major cause of the adcc. this study aims to detect the mutational spectrum of four crystallin genes, cryba1/a3 , crybb1 , crybb2 and crygd in an iranian family. genomic dna was isolated from w...
After the appearance of no-cloning theorem, approximate quantum cloning machines (QCMs) has become a well-studied subject in information theory. Among several measures to quantify performance QCM, single-qudit fidelity and global have been most widely used. In this paper we compute optimal for phase-covariant via semidefinite programming optimization, thereby completing remaining gap previous r...
mycobacterium tuberculosis, the main cause of tuberculosis (tb), has still remained a global health crisis especially in developing countries. tuberculosis treatment is a laborious and lengthy process with high risk of non compliance, cytotoxicity adverse events and drug resistance in patient. recently, there has been an alarming rise of drug resistant in tb. in this regard, it is an unmet need...
a novel single step double positive double negative selection strategy for β-globin gene replacement
introduction: beta thalassemias are a heterogenous group of autosomal recessive disorders, characterized by reduced or absent production of the b-globin chain by the affected allele. transplantation of allogenic hematopoietic stem cells (hsc) is a curative approach but this therapeutic option is not available to the majority of patients. transplantation of genetically corrected autologous hsc i...
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