A rapid isocratic method for determining the total phosphatidylcholine and disaturated phosphatidylcholine levels in lung surfactant preparations by high performance liquid chromatography (HPLC) is described. The analysis was performed on a 3.9 x 300 mm mu-Porasil column with detection by refractive index. The lipids were eluted with a solvent system of chloroform-acetonitrile-methanol-water-85...

Stu~1ies on ,the mechanism of stereoselectivity of chromIUm tncarbonyl catalysts -with model com~ounds provided the basis for the preparation of sImulated fats. These synthetic fats were prepared by .taking advantage of the unique property of chromIUm carbonyl complexes to catalyze hydrogenation of polyunsaturates to cis-~onoui.I­ saturates. Oils simulating the composition of peanut oil were pr...

Acute silica lung injury is marked by alveolar phospholipidosis and type II cell proliferation. Parathyroid hormone-related protein (PTHrP) 1-34 could have a regulatory role in this process because it stimulates phosphatidylcholine secretion and inhibits type II cell growth. Other regions of the PTHrP molecule may have biological activity and can also exert pulmonary effects. This study examine...

Pulmonary hypoplasia and hypertension account for significant morbidity and mortality in neonates with congenital diaphragmatic hernia (CDH). Whether CDH is associated with surfactant dysfunction remains controversial. Therefore, we measured disaturated phosphatidylcholine (DSPC) and surfactant protein (SP)-B concentration in tracheal aspirates and their synthesis rate in infants with CDH compa...

Surfactant is an apically-secreted surface-active material containing primarily disaturated phosphatidylcholine (DSPtdCho) that is released from alveolar epithelia into the alveolus. Surfactant deficiency is an important aspect of inflammatory lung disease and may result from extravasation of serum lipoproteins into the alveolus. We investigated whether one bioactive component of modified lipop...

Stachybotry chartarum, a fungal contaminant of water-damaged buildings commonly grows on damp cellulose-containing materials. It produces a complex array of mycotoxins. Their mechanisms of action on the pulmonary system are not entirely clear. Previous studies suggest spore products may depress formation of disaturated phosphatidylcholine (DSPC), the major surface-active component of pulmonary ...

Differential scanning calorimetry has been applied to the study of some simple model biomembranes, i.e. lipid-water systems, and the endothermic phase transitions which they exhibit when transforming from the gel to liquid crystal phase have been studied. These studies show that: 1. When lipids of the same class, e.g. lecithins with dissimilar chain lengths Cl4 and Cl6 are mixed, a continuous s...