Previously we reported that annexin VI is required for the budding of clathrin-coated pits from human fibroblast plasma membranes in vitro. Here we show that annexin VI bound to the NH2-terminal 28-kD portion of membrane spectrin is as effective as cytosolic annexin VI in supporting coated pit budding. Annexin VI-dependent budding is accompanied by the loss of approximately 50% of the spectrin ...

We studied the localization of low density lipoprotein (LDL) bound to the receptors on the cultured fibroblasts from a patient (MN) with homozygous familial hypercholesterolemia and a defect in internalization of LDL and compared the localization with normal fibroblasts and with those from another internalization-defective cell, GM2408A. Monolayers of cells were cultured with lipoprotein-defici...

median cleft is the midline cleft of the lip. it develops due to incomplete or failed fusion of the median nasal prominence. it can present with minimal deformities such as involvement of the vermilion border, or complex clefting of the midline structures and brain. median clefts are broadly classified as true and false clefts. this case report describes a rare case of median cleft of the upper...

squamous cell carcinoma, a cancer of keratinocytes origin, mostly involves sun-exposed areas of the skin. to study the pathologic and demographic features of this disease, records of 395 patients of squamous cell carcinoma of skin and lip were reviewed. most of the patients (83%) aged 40 years or more at the time of diagnosis. respectively, 74.4% and 75.8% of cases with skin and lower lip carci...

We managed four patients who had neoplastic, posttraumatic, atrophic and postoperative lip deformity, respectively. Case 1: The patient had a hemangioma (17 × 14 mm) confined to the vermilion part of the lower lip. We performed the wedge excision of the tumor and labioplasty. The postoperative appearance and function of the lip were satisfactory. Case 2: The patient had posttraumatic deformity ...

Gorlin's syndrome is an autosomal dominant disorder, mainly characterized by the presence of multiple and early-onset basal cell carcinomas, odontogenic keratocysts and palmoplantar pits. We describe the case of a patient with clinical and imaging features of Gorlin syndrome, and highlight the role of dermoscopy in the early detection of basal cell carcinomas. In addition, we discuss the dermos...

Most knowledge of clathrin-mediated endocytosis has been gained by biochemical fractionation and in vitro assays. Recently, the study of endocytosis has extended into the living cell. The tracking of individual clathrin-coated pits and vesicles (CCPs and CCVs) has provided new insight into understanding the dynamic nature of CCPs. The use of total internal reflection fluorescence microscopy (TI...

Objective(s)CpG oligodeoxynucleotides (CpG ODNs) have been shown to have potent immunostimulatory adjuvant activity for a wide range of antigens. Due to susceptibility of phosphodiester CpG ODNs (PO CpG) to nuclease degradation, nuclease-resistant phosphorothioate CpG ODNs (PS CpG) were currently utilized in an in vivo model. In this study, according to some recently reported drawbacks with PS ...