A novel analytical procedure based on chemiluminescence (CL) detection was described for the determination of lysozyme at ng ml(-1) level by using controlled-reagent-release technology in a flow injection system. The analytical reagents involved in the CL reaction, including luminol and periodate, were both immobilized on the anion-exchange resins in the flow injection system. Through water inj...

Total antioxidant capacity (TAC) of several samples of red and white wines was investigated by means of a new analysis method of flow injection with chemiluminescence detection ((FIA-CL). The applied method is based on a chemiluminescence reaction between luminol and hydroxyl radicals generated by the hydrogen peroxide in the presence of Co(II) ions complexed with ethylene diamine tetraacetic a...

A new, simple, rapid and sensitive batch chemiluminescence (CL) method for determination of six fluoroquinolones (ciprofloxacin, gatifloxacin, levofloxacin, lomefloxacin HCl, ofloxacin and sparfloxacin) is proposd. The method is based on the CL generated during the oxidation of luminol by N-bromosuccinimide (NBS) in alkaline medium. The determination of the studied drugs is based on their inhib...

The increasing use of metal-based nanoparticle products has raised concerns in particular for the aquatic environment and thus the quantification of such nanomaterials released from products should be determined to assess their environmental risks. In this study, a simple, rapid and sensitive method for the determination of size and mass concentration of gold nanoparticles (AuNPs) in aqueous su...

We conducted this study to determine whether physiological changes in potassium concentration affect free radical formation by vascular cells. We assessed the effects of potassium on reactive oxygen species formed by cultured endothelial and monocyte/macrophage cells or freshly isolated human white blood cells by cytochrome c reduction or luminol chemiluminescence, respectively. Reducing potass...

Cyanate (CNO-) forms spontaneously in solutions containing urea, and is present in urine and the body fluids of uraemic patients. We have explored the possibility that CNO- might be one of the unknown substances responsible for the reported impairment, by urine and uraemic plasma, of neutrophil oxidative metabolism (especially as measured by luminol-enhanced chemiluminescence). Luminol-enhanced...

The presence of sulfonamide (SA) residues in foods is largely due to the raising of animals with sulfonamide antibiotics added or polluted feedstuff. In this paper, a sensitive method was developed for the determination of the residues of three sulfonamides in animal-derived food; the SAs include sulfadimidine (SDD), sulfadiazine (SDZ), and sulfathiazole (STZ). The method is based on capillary ...

A method has beed developed for the analysts of cobalt In natural waters by catlon-exchange llquld chromatography udng lumlnol chemllumlnescence detectlon. Cobalt can be detemrkred dlrectty In freshwater samples on 500-pL samples wlth a detectlon llmlt of 20 pmol/kg; larger samples provide proportknately bwer detectlon #mlts. Seawater samples can be analyzed on lOOmL samples followlng APDC solv...

Um procedimento empregando detecção quiluminescente para determinação de carbaril em águas naturais utilizando acetilcolinesterase e colina oxidase é descrito. O módulo de análise projetado para implementar o processo de multicomutação controlado por microcomputador foi constituído por cinco válvulas solenóides, duas colunas com enzimas imobilizadas em esferas de vidro e cela de fluxo para dete...

A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the antiAPnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The lum...