Luminol (5-amino-2,3,-dihydrophthalazine-1,4-dione) is synthesized and its chemiluminescent properties are explored. Both spectral and physical data indicate that the luminol is sufficiently pure, despite a low yield. Chemiluminescence of luminol is observed in the presence of H2O2 and an oxidant enhancer. The quantum yield of the luminol chemiluminescence is determined to be moderate (0.10 ± 0...

We report a study on chemiluminescence-based chemical analyses using luminol molecules covalently attached to 10 nm diameter gold nanoparticles (GNPs). Chemiluminescence (CL) has been systematically studied under two schemes by varying the concentrations of luminol-labeled GNPs and [Fe(CN)6](3-) catalyst, respectively. The CL signal of luminol-labeled GNPs is enhanced by 5 to 10 times compared ...

The chemiluminescence of the luminol-H2O2-horseradish peroxidase system is increased by fluorescein. Fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. The maximum intensity and the total chemiluminescence emission (between 380 and 580 nm) of luminol with fluorescein was more than th...

The most efficient and commonly used electrochemiluminescence (ECL) emitters are luminol, [Ru(bpy)3 ]2+ , and derivatives thereof. Luminol stands out due to its low excitation potential, but applications are limited by its insolubility under physiological conditions. The water-soluble m-carboxy luminol was synthesized in 15 % yield and exhibited high solubility under physiological conditions an...

A multi-functional luminol-reduced Pt@Au hybrid flower-like nanocomposite (luminol-Pt@AuNF) which not only acts as an efficient signal probe but also constitutes a pseudobienzyme amplifying system with choline oxidase (ChOx) was firstly synthesized and applied to the construction of a solid-state luminol electrochemiluminescence (ECL) immunosensor for cardiac troponin I (cTnI) detection.

Horseradish peroxidase (HRP) encapsulated in liposomes was directly detected by using luminol chemiluminescence (CL) with H2O2 without lysis of liposomes. At a low concentration of H2O2, the initial rate of HRP-catalyzed luminol CL in liposomes was slower than that of HRP-catalyzed luminol CL in a lipid-free bulk solution. The decrease in the initial rate of the CL reaction in liposomes was due...

Three chemicals (luminol, f luorescein, and Bluestar) are photographically compared. The results demonstrate that Bluestar performs as well or better than luminol and f luorescein. Introduction Detecting and photographing latent bloodstains at a crime scene can be challenging. The ease through which a presumptive chemical test is prepared, administered, and viewed is the first obstacle. Photogr...

Luminol and LCV are commonly used reagents to develop latent bloodstains on evidence and at crime scenes. Luminol was first used to detect latent bloodstains in 1937 (1). Since that time the use of luminol has become very popular with many law enforcement agencies. The application of luminol creates a blue/green color chemiluminescence from its reaction with hemoglobin. Observation and subseque...

This study was designed to test the following factors involved with processing luminol treated bloodstained evidence: 1) The reactivity of other presumptive chemical color tests, phenolphthalin (PT) and tetramethylbenzidine (TMB), following the application of the light emitting luminol presumptive test. 2) The effect of different cleanings of various bloody substrates on the luminol test. 3) Th...