Abstrak. Keberhasilan ekstraksi DNA sangatlah penting dalam proses investigasi genetika molekuler. Penggunaan sampel biasanya berupa organ tanaman muda karena diketahui dapat menghasilkan ekstrak yang bagus. Kayu dan kulit kayu juga berpotensi sebagai sumber sifatnya mudah didapat. Namun pengujian efektifitasnya untuk spesies Pinus merkusii belum pernah dilaporkan. Tujuan penelitian membandingk...

efficient tuberculosis treatment requires the use of good quality medicines. the present study developed a method of dosage by high performance liquid chromatography (hplc) coupled with the dissolution of a combination of fixed doses of four anti-tuberculous (isoniazid, pyrazinamide, ethambutol hydrochlorate, rifampicine). the elaborated protocol was thereafter used to assess the quality of med...

INTRODUCTION Anti GAD (antibodies on glutamic acid decarboxylase) and anti-IA2 antibodies (against tyrosine phosphatase), today, have their place and importance in diagnosis and prognosis of Type 1 diabetes. Huge number of patients with diabetes mellitus type 1 have these antibodies. Insulin antibodies are of critical importance in diagnosis of diabetes mellitus type 1 for pediatric population....

Newbonr mice were treated from the day of birth with either bovine gamma globulin or anti-mu chain sera. The latter was administered using a protocol known to produce suppression of gammaM, gammaG, and gammaA production. Subsequent immunization with ovalbumin (OA) in alum was attempted to see if suppression of gammaM, gammaG, and gammaA classes of antibody would also be accompanied by suppressi...

Rapid counter-immunoelectrophoresis (CIE) and radioimmunoassay (RIA) methods for detecting antibody to hepatitis B core antigen (anti-HBc) were used to screen nearly 8000 blood donors, including 919 prisoners. The prevalence of anti-HBc in prisoner donors (3.4%) was significantly higher than that in other donors (0.7%). The three HBsAg positive donors in the series were all anti-HBc positive an...

INTRODUCTION We aimed to evaluate the prevalence of "anti-HBc alone" among Syrian blood donors, highlighting the possibility of representing occult HBV infection. METHODOLOGY Sera of 3,896 healthy blood donors were tested for both HBsAg and anti-HBc. HBsAg-negative, anti-HBc-positive samples were further tested for the antibodies to HBsAg (anti-HBs), and "anti-HBc alone" sera were tested for ...

1. the most important challenge in diagnosis of celiac disease is not-performing the diagnostic tests in suspected persons. because of multi-organ damage and multiple manifestations of disease, diagnosis of celiac disease may be delayed. it seems general physicians should be aware about uncommon presentations of disease and indications of celiac tests. 2. the second most important challenge is ...

BACKGROUND Infectious disease marker testing is obligatory for the release of human tissue for transplantation. Most CE-marked tests are not validated for postmortem blood. In a previous study we have validated the testing for anti-HIV-1/2, anti-HCV, HBsAg, and anti-HBc. Here, we present the validation of testing for antibodies against T. pallidum, which is the last marker obligatory for tissue...