The perfusion mode of a continuous cell culture bioreactor was modified to establish a closed loop system. Eighty percent of the spent medium was re-used twice. The medium cycle bioreactor unit was operated sterile and uncomplicated without a technical retention system for the high molecular weight substances. Therefore, only 20% of the actual medium was necessary to run the recycling process. ...

Introduction and objective: The appearance of resistance to anti-tuberculosis drugs has generated research to find new and more effective drugs. Löwenstein-Jensen medium (LJ) is frequently used for culturing strains of Mycobacterium tuberculosis. A group of antimicrobial substances used in treating tuberculosis is sensitive to heat and cannot be used on LJ medium. Research now aims to setup a m...

Background and objectives: Hawthorn as an ethno-pharmaceutical plant has been consumed for 2,500 years. Flowers, leaves and fruits of hawthorn are rich in antioxidant and polyphenols and are popular in the treatment of congestive heart failure. Methods: In the present study, by using various media and plant hormones the regeneration and callogenesis capacity of...

large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as fi lling materials. the aim of this study was to evaluate the cytotoxic effect of a new copaiba oil-based root canal sealer (biosealer [bs]) on osteoblast-like osteo-1 cells. materials and methods: the experimental groups were formed according to the culture medium cond...

This study was designed to provide further information on mouse and human embryo development in alpha-modified minimum essential medium (alphaMEM). First, we compared the development and implantation potential of murine in-vitro fertilized (IVF) embryos cultured in alphaMEM, in the presence and absence of co-culture cells. No significant difference was observed in blastocyst rate between alphaM...

Although three-dimensional testicular cell cultures have been demonstrated to mimic the organization of the testis in vivo and support spermatogenesis, the optimal culture conditions and requirements remain unknown. Therefore, utilizing an established three-dimensional cell culture system that promotes differentiation of pre-meiotic murine male germ cells as far as elongated spermatids, the pre...

E. A. Ghazy, M.G. Mahmoud, M. S. Asker, M. N. Mahmoud, M. M. Abo Elsoud and M. E. Abdel Sami Microbial Biotechnology Department, National Research center, Dokki, Cairo, Egypt [email protected] Abstract: Sulfate-reducing bacteria (SRB) represent a class of anaerobic microorganisms that conduct dissimulatory sulfate reduction to obtain energy. The present study aimed to detect and control SRB...