A 64-year-old Bangladeshi female presented to her primary care physician with a tender right breast lump that had been present for 4-5 days along with subjective fevers and malaise. Initial biopsy revealed granulomas, but Ziehl-Neelsen and Gram stain were negative for TB so antibiotics were prescribed for abscess until culture came positive for tuberculosis. She was started on triple therapy fo...

Histological examination of lymph-node biopsy specimens in 12 patients with erythema nodosum leprosum showed almost complete replacement of the node by lepromatous granuloma, together with considerable polymorph infiltration. Ziehl-Neelsen staining demonstrated numerous Mycobacterium leprae present in the nodes. The majority of these patients were very ill, and responded to prednisolone or cort...

in this study prevalence of parasites in 118 samples (62 foxes and 56 jackals) was investigated in ilam province of iran, in 2010-2013, situated in the western part of the national capital of iran. the nematodes which were cleared in lactophenol, cestodes and acanthocephalans were stained using acetocarmine and haematoxylen, respectively for identification of the parasite species. cryptosporidi...

cryptosporidium parvum is a zoonotic protozoan parasite with a wide range of vertebrate hosts. the present study was conducted to determine the prevalence of cryptosporidium parvum in urban and rural dogs of the ahvaz area. faecal samples were collected randomly from 93 dogs between may 2005 and september 2007. the studied dogs were divided into two groups (urban and rural) and based on age int...

conclusions the results of the study showed a high prevalence of giardia lamblia and blastocystis hominis in rural areas compared to urban regions. therefore it is necessary to promote the public health awareness in the rural population, in order to reduce the frequency of parasitic infections. background nowadays, parasitic infections are a major health problem throughout the world, particular...

The detection of genetically modified (GM) materials in food and feed products is a complex multi-step analytical process invoking screening, identification, and often quantification of the genetically modified organisms (GMO) present in a sample. "Combinatory qPCR SYBRGreen screening" (CoSYPS) is a matrix-based approach for determining the presence of GM plant materials in products. The CoSYPS...

The principles and techniques involved in fluorescence microscopy as related to the demonstration of acid-fast bacilli are discussed. The auramine-rhodamine staining method, for both tissue and bacteriological preparations, is described as well as the necessary equipment for both microscopy and photomicroscopy. Fluorescence staining is no more difficult nor time consuming than a Ziehl-Neelsen s...