background: cryptosporidium is a protozoan parasite causes cryptosporidial diarrhea , which is typically a short-lasting benign infection, but can become severe and non-resolving in immunocompromised individuals. the aim of the present study was to determinate the prevalence of cryptosporidium infection in livestock and humans that were in contact with livestock in hamadan district, iran. metho...

Although inexpensive and easy to perform, the negative staining technique of Heine for the detection of Cryptosporidium spp. has been largely neglected. The lack of familiarity with the negative staining technique, the misconception that a phase-contrast microscope is indispensible and inferior results due to Köhler-illumination, are possible explanations for its low popularity. While the modif...

Modified Ziehl–Neelsen staining for Mycobacterium leprae is a time-tested technique in the evaluation of leprosy. The helps to classify clinical types leprosy into those with low and high bacillary load. It also useful assessing response therapy, identifying defaulters, differentiating relapse from late reversal reactions. In this net educational video, we demonstrate taking an ear lobe smear p...

We compared the sensitivity and specificity of a modified Ziehl-Neelsen (modified-ZN) staining method for acid-fast bacilli (AFB) with that of the standard Ziehl-Neelsen (standard-ZN) staining method, using culture results with Mycobacterium tuberculosis as the "gold standard." The sensitivity (72%; 101 of 140) of the modified-ZN staining method, which uses 0.3% carbol fuchsin, was significantl...

Negative staining technique of Heine is an easy, inexpensive, and rapid way of screening for coccidian parasites of the intestinal tract. But its use as a routine technique for screening of Cryptosporidium, Cyclospora, and Cystoisospora is restricted due to its sensitivity being lower than the gold standard method of modified Ziehl-Neelsen staining. This paper emphasises the modification of ori...

AIM The aim of this study is evaluation of molecular assay and the standard staining method. BACKGROUND Cryptosporidium is a protozoon from coccidian subclass, which is one of the most important causes of diarrhea in children and immunocompromised individuals around the world. Diagnosis and treatment are necessary for mentioned cases. Usual diagnostic method for this parasite is fecal smear p...

 SUMMARY In an investigation in summer· 1993 in health centers, 431 faecal samples from children with diarrhea were examined. In 14 (6 girls and 8 boys) 3.25 percent with modified Ziehl -Neelson Staining technique Oocyst of cryptosporidium has been demonstrated and the results discussed.

Poor laboratory equipment and few human resources have made it difficult to implement microscopic diagnosis of pulmonary tuberculosis (TB) on a large scale basis worldwide. Three hundred sputum samples from patients in Cameroon were studied by using the CyScope®, a new light-emitting, diode-based, fluorescence microscope, to compare auramine-rhodamine fluorescence with the conventional Ziehl-Ne...

The prevalence of cryptosporidial infection among captive wild animals and birds in Sanda Kyarimi Park, Maiduguri, north-eastern Nigeria, was evaluated by faecal examination for oocysts using three different staining techniques: the modified Ziehl-Neelsen, Giemsa, and Safranin-methylene blue. A total of 66 captive wild animals and birds were examined based on age and sex differences. Fifteen (2...