نتایج جستجو برای: monolayer cell culture
تعداد نتایج: 1881478 فیلتر نتایج به سال:
Autologous chondrocyte implantation (ACI) is a method of cartilage repair. To improve the quality of regenerated tissue by ACI, it is essential to identify surface marker expression correlated with the differentiation status of monolayer expanded human articular chondrocytes and to define the index for discriminating dedifferentiated cells from monolayer expanded human articular chondrocytes. N...
Introduction: Adipose-derived mesenchymal cells (AMCs) are a promising cell source for orthopaedic tissue engineering applications due to their accessibility and multi-lineage potential. However, future use in bone and cartilage regeneration requires development of successful strategies for driving AMCs to osteogenic and chondrogenic lineages. Recent work has demonstrated that bone morphogeneti...
Reliable model systems are needed to elucidate the role cancer stem cells (CSCs) play in pediatric brain tumor drug resistance. The majority of studies to date have focused on clinically distinct adult tumors and restricted tumor types. Here, the CSC component of 7 newly established primary pediatric cell lines (2 ependymomas, 2 medulloblastomas, 2 gliomas, and a CNS primitive neuroectodermal t...
High content imaging (HCI) is a multiplexed cell staining assay developed for better understanding of complex biological functions and mechanisms of drug action, and it has become an important tool for toxicity and efficacy screening of drug candidates. Conventional HCI assays have been carried out on two-dimensional (2D) cell monolayer cultures, which in turn limit predictability of drug toxic...
Steroid hormones affect the growth of many tumor cells both in vivo and in vitro. Growth of cells in vitro can be studied as either anchorage dependent (monolayer culture) or anchorage independent (suspension culture), and in each case, steroid hormones can alter log-phase proliferation rate, saturation density, or cell morphology. Results presented here demonstrate that different steroid hormo...
conclusions our underlying hypothesis is that recapitulating the three-dimensional early embryonic structure, in order to reproduce better in vitro the three-dimensional morphogenetic-like re-arrangements, would improve cells differentiation, when in vivo transplanted; moreover, it could be used as a simplified cancer disease model and reliable drug evaluation method as well. results the proces...
Introduction: The Swarm rat chondrosarcoma is a model system for the study of chondrocyte metabolism and matrix synthesis, especially proteoglycans. Chondrocytes, when cultured in monolayer tend to dedifferentiate into fibroblasts over time, yet parallels are often drawn between in vitro monolayer culture and the in vivo state of cartilage, even though the cells are phenotypically different. A ...
Airway epithelial cells (AEC) are critical components of the inflammatory and immune response during exposure to pathogens. AECs in monolayer culture and differentiated epithelial cells in air-liquid interface (ALI) represent two distinct and commonly used in vitro models, yet differences in their response to pathogens have not been investigated. In this study, we compared the transcriptional e...
Two epithelial cell lines were established from separate pools of cells that were harvested by a sequential proteolytic treatment of a single specimen of rat transplantable colonic carcinoma. The cells were small (10-micrometer diameter) and cuboidal and displayed desmosomes and light junctions. Both cell lines grew rapidly with population-doubling times of 20 to 22 hr, were near diploid in chr...
This paper presents a novel method for tracking and characterizing adherent cells in monolayer culture. A system of cell tracking employing computer vision techniques was applied to time-lapse videos of replicate normal human uro-epithelial cell cultures exposed to different concentrations of adenosine triphosphate (ATP), acquired over a 20 hour period. Subsequent analysis, comprising feature e...
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