A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3% (w/v) sucrose. The rate of multip...

this research was aimed to investgate the effect of light quality on micropropagation of pear rootstock oh×f333. first, single nodes explants were cultured in modified ql medium containing ba at four levels (0, 0.5, 1, and 2 mg/l-1) and iaa at three levels (0, 0.1, and 1 mg/l-1) and were evaluated for shoot proliferation. then, plantlets subcultured in modified ql medium containing 1 mg/l-1 ba ...

This investigation was carried out at the Tissue Culture Lab., Biotechnology Research Center (BTRC) in Tripoli, Libya 2009 to establish a micro propagation protocol for two hybrids of Tomato which several factors were studied including effect concentration growth regulator on shoot multiplication, and rooting explant MS media. Shoot tips cultured into (MS medium) with different concentrations b...

in vitro propagation of plant has played a very important role in rapidmultiplication of cultivars with desirable traits and production of healthy and disease-free plants. in the present investigation, the objectives were to optimize the micropropagation of rose hybrid ‘black baccarat’ cultivar. in proliferation step, the nodal segments (1.5 cm) was cultured on both liquid and solid media (ms, ...

The induction of rooting in microshoots of Zingiber officinale cvs. Suprava, Turia local, Suruchi and V3S18 was achieved on half-strength basal Murashige and Skoog's medium supplemented with 0.5-1.0 mg/l either indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) and 2% (w/v) sucrose within 7-9 days of culture. Rooting was inhibited when the microshoots were cultured under higher concentra...

In vitro rooting response and indole-3-acetic acid (IAA) levels were examined in two genetically related dwarfing apple (Malus pumila Mill) rootstocks. M.26 and M.9 were cultured in vitro using Linsmaier-Skoog medium supplemented with benzyladenine (BA), indole-3-butyric acid (IBA), and 1,3,5-trihydroxybenzoic acid (PG). Rooting response was tested in Lepoivre medium supplemented with IBA and P...

The present study investigates the effects of different concentrations, as well as type of plant growth regulators (PGRs) and medium (MS, Duchefa) on the growth and development of Centella asiatica in semi-solid culture. In addition, a protocol for successful sterilization of C.asiatica explants prepared from field-grown plants highly exposed to fungal and bacterial contamination was determined...

An emerging paradigm is that root traits that reduce the metabolic costs of soil exploration improve the acquisition of limiting soil resources. Here, we test the hypothesis that reduced lateral root branching density will improve drought tolerance in maize (Zea mays) by reducing the metabolic costs of soil exploration, permitting greater axial root elongation, greater rooting depth, and thereb...