Endothelial progenitor cell (EPC) dysfunction is an important mediator of vascular disease in diabetes. We aimed to elucidate the mechanism of adhesion of EPC to diabetic and non-diabetic arteries and to study the effect of the anti-diabetic drug pioglitazone. Peripheral blood mononuclear cells were isolated from healthy donors. Human internal mammary arteries (HIMA) were isolated from patients...

Various defects in platelet function have been reported as being associated with multiple myeloma. In 30 myeloma patients and 15 healthy controls, we investigated platelet survival using in vitro labeling of autologous platelets with 111indium-oxine and measuring the in vivo kinetics of the radioisotope. Significantly shortened platelet half-life in patients averaged 73 hours, while platelet ha...

The feasibility of imaging the inflammatory response to acute transmural myocardial infarction in man using biologically active indium-111 (111In)-labeled autologous leukocytes was assessed in 36 patients. Indium-111 leukocytes (approximately 500 microCi) were injected i.v. 18-112 hours after the onset of chest pain. Cardiac imaging was performed 24 hours later with a mobile gamma camera. Twent...

Indium-111 (111In) and technetium-99m (99Tcm) Stealth liposomes were compared with 111In- and 99Tcm-labelled white blood cells (WBC) in experimental infection in a rabbit model. Preformed polyethylene glycol-coated liposomes and separated WBC were radiolabelled with either 111In-oxine or 99Tcm-hexamethylpropyleneamine oxime (99TcM-HMPAO). After the intravenous administration of one of the four ...

as a synthetic precursor of arylsulfonyl 8-hydroxyquinoline derivatives. It is also a key-intermediate for the synthesis of quinolinol metal compounds,2 which were sought for development as potential electroluminescence devices, or novel oxine and its derivatives3 as antibacterial and antifungal agents. During the course of our investigation concerning the synthesis of novel thiaoxa-donor podan...

We report the results of imaging with labelled white cells in 52 patients before the revision of 54 cemented joint prostheses at which the diagnosis of infection was made from biopsies. Twenty-five hips were imaged with 111In-oxine-labelled cells; 20 hips and 11 knees were imaged with 99mTc-hexamethylpropylene-amineoxime-labelled cells. Of these, 13 hips and five knees proved to be infected. Th...

background: the incorporation of thallium-201 into 8-hydroxyquinoline was targeted for cell labeling due to interesting physical properties and wide availability of this nuclide as a single photon emission computed tomography (spect) radionuclide. materials and methods: thallium-201 (t1/2=3.04 d) in tl+ form was converted to tl3+ cation in presence of o3/6m hcl and di-isopropyl ether, controlle...

Two spectrophotmetric methods based on combined redox - ligand exchange reactions were developed for the determination of captopril and penicillamine in pure form and in their dosage forms. The first method is based on attenuating the absorbance of a ternary complex: silver (I) - bromopyrogallol red - phenanthroline in a buffer solution of pH6-8. The method has the concentration ranges 2-10 μg ...

Tissue factor (TF) antagonists targeting the factor VII (FVII) binding domain have been shown to interrupt acute vascular thrombus formation without impairing haemostasis in non-human primates. In this study, we evaluate whether a human/mouse chimeric monoclonal antibody (ALT-836, formerly known as Sunol-cH36) blocking the factor X/factor IX (FX/FIX) binding site of tissue factor could achieve ...

Simple and novel spectrophotometric method is described for simultaneous determination of aluminium and iron. The method is based on the metal ions complexes formed by aluminium and iron with mixed chromogenic reagents of 1, 10 phenanthroline and 8-hydroxyquinoline (oxine). The derivatives have absorption maxima at 400 nm and 510 nm respectively in the presence of cetyltrimethyl ammonium bromid...