A ll cells are divided into distinct subcellular compartments, each with its own defining set of proteins. A fundamental problem in molecular cell biology is to understand how proteins that are synthesized on ribosomes in the cytoplasm reach their proper intracellular address. This process, usually called protein sorting or protein trafficking, is understood to involve information encoded in th...

A microfluidic immunoassay based on Europium nanoparticles has been developed for rapid and sensitive detection of HIV-1. The core polymer microfluidic device contains immunoreactors anchored with monoclonal capture antibodies highly specific to p24, the capsid protein of HIV-1. To report the capturing events, biotinylated secondary antibody followed by streptavidin-conjugated Europium nanopart...

Six new members of the yeast p24 family have been identified and characterized. These six genes, named ERP1-ERP6 (for Emp24p- and Erv25p-related proteins) are not essential, but deletion of ERP1 or ERP2 causes defects in the transport of Gas1p, in the retention of BiP, and deletion of ERP1 results in the suppression of a temperature-sensitive mutation in SEC13 encoding a COPII vesicle coat prot...

Human erythrocytes bearing electroinserted full-length CD4 (RBC-CD4) can bind and fuse with a laboratory strain of human immunodeficiency virus type 1 (HIV-1) or with T cells infected by HIV-1. Here we show that RBC-CD4 neutralize primary HIV-1 strains in an assay of cocultivation of peripheral blood mononuclear cells (PBMC) from HIV-1 -infected persons with uninfected PBMC. RBC-CD4 inhibited v...

To better understand the qualitative features of effective human immunodeficiency virus (HIV)-specific immunity, we examined the TCR clonal composition of CD8(+) T cells recognizing conserved HIV p24-derived epitopes in HLA-B*5701-positive long-term nonprogressors/elite controllers (LTNP/EC) and HLA-matched progressors. Both groups displayed oligoclonal HLA-B5701-restricted p24-specific CD8(+) ...

This report is an analysis of results reported to the CDC by laboratories participating in the MPEP after they performed p24 antigen determinations on HIV-1 performance evaluation samples shipped to them March 1, 1999. Testing results were reported by 170 (91.9%) of the 185 laboratories that received sample panels. One laboratory returned results more than 2 weeks after the cut-off date and one...

Recently, antimicrobial peptides have been introduced as potent antibiotics with a wide rangeof antimicrobial activities. They have also exhibited other biological activities, including antiinflammatory,growth stimulating, and anti-cancer activities. In this study, an analog of MagaininII was designed and produced as a recombinant fusion protein. The designed sequence containe...

····························· P3 Introduction ·····························P4-8 Material and Methods ·························P9-12 Results ······························· P13-19 Discussion and Conclusions ·······················P20-24 Acknowledgements ·························· P24 References ····························· P25-26

develops and do not rise again until late in the course of HIV infection. There are few cases when HIV antibodies are not detectable viz., people who have a severely impaired immune system may take longer to develop HIV antibodies and those who have taken post‐exposure prophylaxis (PEP). If PEP does not successfully prevent HIV infection, the presence of the PEP drugs may still reduce HIV repli...