Tissue engineered constructs have the potential to respond unmet medical need of treating deep osteochondral defects. However, current tissue engineering strategies struggle in attempt create patterned with biologically distinct functionality. In this work, a developmentally-inspired modular approach is proposed, whereby cartilaginous organoids are used as living building blocks. First, hierarc...

The inner cell mass (ICM) of the implanting mammalian blastocyst comprises two lineages: the pluripotent epiblast (EPI) and primitive endoderm (PrE). We have identified platelet-derived growth factor receptor alpha (PDGFRα) as an early marker of the PrE lineage and its derivatives in both mouse embryos and ex vivo paradigms of extra-embryonic endoderm (ExEn). By combining live imaging of embryo...

Arylamine N -acetyltransferases (NATs) catalyse the acetylation from acetyl-CoA of arylamines and hydrazines. There are two human isoenzymes which show polymorphism, and both enzymes are involved in the activation and detoxification of environmental carcinogens and teratogens. The two human isoenzymes NAT1 and NAT2 show different tissue distribution, with human NAT2 being found in liver and int...

background: galectin-3 (gal-3), a β-galactoside-binding lectin, is a multifunctional lectin that involves in a number of critical biological processes. objective: the purpose of this study was to investigate the expression pattern of gal-3 in mouse endometrium during estrus phase of estrous cycle and pre-implantation. materials and methods: in this experimental study 42 nmri female mice were di...

In patients with end-stage left ventricular (LV) heart failure who receive LV assist device (LVAD) implantation, right ventricular (RV) failure represents a possible critical complication that heavily affects morbidity and mortality. Several clinical, laboratory, hemodynamic, and echocardiographic variables have been found to be associated with RV failure occurrence after surgery. Different mod...

The extraction of intact RNA from oocyte is quite challenging and time-consuming. A standard protocol using commercial RNA extraction kit, yields a low quantity of RNA in oocytes. In the past, several attempts in getting RNA for gene expression study ended up with a few different modified methods. Extraction of high-quality RNA from oocyte is important before further downstream analyses such as...

Background: Diabetes is a metabolic disease caused by a deficiency in the pancreatic secretion of insulin and/or by the inability of tissues to efficiently respond to insulin, events that cause hyperglycemia and affect all organs.Tissue alterations caused by diabetes affect different organic systems , including the male reproductive system. Reproductive dysfunction is one of the common secondar...

This study evaluated the influence of intra-operative soft tissue balance on post-operative range of motion in patients undergoing posterior-stabilized total knee arthroplasty with navigation. After implantation of all components in 31 consecutive patients, the joint component gap was measured manually with the knee at 0°, 90°, and 120° as guided by a navigation system. We designated soft tissu...

BACKGROUND After fertilization, embryo development involves differentiation, as well as development of the fetal body and extra-embryonic tissues until the moment of implantation. During this period various cellular and molecular changes take place with a genetic origin, e.g. the elongation of embryonic tissues, cell-cell contact between the mother and the embryo and placentation. To identify g...