The proportional bias of four methods frequently used for determining low concentrations of protein was evaluated with human serum protein fractions (Cohn Fractions II, III, IV, and V). Each fraction was assigned a protein concentration value as determined by the biuret method, calibrated with purified human serum albumin monomer. All four methods (Coomassie Brilliant Blue dye-binding, the meth...

When rabbits are injected with tissue proteins, precipitin antibodies for blood serum proteins appear in the serum in addition to precipitins for the tissue proteins (5, 6, 2, I). There are three methods by which the former can be removed; namely, absorption in vitro, absorption in vivo, and allowing sufficient time to elapse following the injection for the exhaustion of blood proteins from the...

The intestinal peptide transporter PEPT1 mediates the uptake of di- and tripeptides derived from dietary protein breakdown into epithelial cells. Whereas the transporter appears to be essential to compensate for the reduced amino acid delivery in patients with mutations in amino acid transporter genes, such as in cystinuria or Hartnup disease, its physiological role in overall amino acid absorp...

Fatty acid transport protein-4 (FATP4) is the major FATP in the small intestine. We previously demonstrated, using in vitro antisense experiments, that FATP4 is required for fatty acid uptake into intestinal epithelial cells. To further examine the physiological role of FATP4, mice carrying a targeted deletion of FATP4 were generated. Deletion of one allele of FATP4 resulted in 48% reduction of...

Due to inherent complexity active transport presents a landmark hurdle for oral absorption properties prediction. We present a novel approach carrier-mediated drug absorption parameters calculation based on entirely different paradigm than QSPR. We capitalize on recently emerged ideas that molecule activities against a large protein set can be used for prediction of biological effects and perfo...

Sodium absorption in the mammalian small intestine occurs predominantly by two primary pathways that include Na/H exchange (NHE3) and Na-glucose cotransport (SGLT1) on the brush border membrane (BBM) of villus cells. However, whether NHE3 and SGLT1 function together to regulate intestinal sodium absorption is unknown. Nontransformed small intestinal epithelial cells (IEC-18) were transfected wi...

Treatment of adrenal iron-sulfur protein (adrenodoxin) with urea and guanidine HCI results in a deterioration reaction that proceeds through at least two intermediate stages yielding the completely denaturated, colorless protein. The first transient state exhibits a circular dichroism maximum at 470 rnp which is shifted to a longer wave length than that of the native protein (440 mp) ; at 470 r...

background: this study aimed to assess several biochemical and oxidative stress parameters before and after radioiodine therapy in patients with well-differentiated thyroid cancer who consumed a low iodine diet and withdrawal of levothyroxine after total thyroidectomy. methods: we enrolled 40 candidates for radioiodine therapy. blood sampling was performed prior to as well as 72 h after consump...

Numerous workers have established a connexion between the metabolism of magnesium and various dietary factors. Thus Colby & Frye (1951 b) working with young rats on a low-Mg, low-calcium diet observed reduction in blood Mg levels and increase in mortality rate when the casein content of the diet was increased from 24 to 50 yo. Increased intake of Ca salts or of phosphates has been reported to a...

This study aimed at determining the proximate, functional, and physicochemical properties of Basella alba whole flour (BWF) protein concentrate (BPC) for use as food ingredients with a view to improve their utilization by producing from its content which will serve cheap but abundant source protein. OAU farm provided fresh leaves. The stalk was quickly cut off, leaves were cleaned under running...