AIM To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and ISolate. METHODS This prospective study used semen specimens from 22 patients. Specimens from nine patients were processed by both PureSperm density gradient and swim-up met...

high ambient temperature is the major constraint on buffalo productivity. the aim of this study was to evaluate the reproductive performance and thyroid gland function in winter and summer seasons in khuzestan buffalo bulls. six male indigenous buffaloes of khuzestan with nearly the same age (2-3 years old) and weight were used. semen and blood samples through jugular vein were collected, every...

OBJECTIVE To develop a model to predict transmission of HIV-1 from men to women. DESIGN HIV-1 in seminal plasma, and endocervical CCR5 receptors were correlated with epidemiological studies of HIV-1 transmission to develop a probabilistic model. SETTINGS Semen samples were collected from patient subjects in Seattle Washington, Chapel Hill, North Carolina, and St. Gallen, Switzerland. Endoce...

To evaluate and optimize the sperm separation efficiency of a novel silane-coated silica bead (Puresperm), serial studies were carried out to compare the various sperm parameters between: (i) three-layer (90%-70%-40%) Puresperm and three-layer (90%-70%-40%) conventional polyvinylpyrrolidone (PVP)-coated silica bead (Percoll) gradients; (ii) three-layer (90%-70%-40%) and two-layer (90%-45%) Pure...

The purpose of the present study was to assess the effect of modified camel milk (MCAM) and skim cow milk (SCOM) on semen quality of INRA180 and Boujaâd ram breeds, stored at 15°C for up to 24 hours. Semen samples were collected from eleven mature rams (6 from INRA180 and 5 from Boujaâd rams) and extended in SCOM vs MCAM, to reach a final concentration of 0.8×109 spermatozoa/mL, and stored at 1...

Hypertonic solutions play an important role in the treatment of tissue edema. Following the induction of experimental vasogenic brain edema by occlusion of the common carotid arteries in eight rabbits, the effect of hypertonic lactated Ringer's solution (subjects), vs. isotonic lactated Ringer's solution (control), was studied on blood pressure, electrolyte (sodium, potassium, and calcium) ...

Although geographic variation in semen quality has been reported, this is the first study in the United States to compare semen quality among study centers using standardized methods and strict quality control. We evaluated semen specimens from partners of 512 pregnant women recruited through prenatal clinics in four U.S. cities during 1999-2001; 91% of men provided two specimens. Sperm concent...

The objective of the present study was to estimate heritabilities and genetic correlations for semen and litter size traits using full animal model analyses. Litter size data were available from 28,485 Czech Large White and 10,410 Czech Landrace sows farrowing between 1995 and 2008. The number of litter records was 91,922 and 30,937, respectively. Records on semen traits (37,137 ejaculates from...

The objective of this study was to specify the effects of centrifugation and different surface of soybean lecithin-based extender on post-thaw Sperm quality of Ghezel ram semen. Semen samples were gathered from 5 mature Ghezel ram two/week for 3 weeks. After initial evaluation, the approved semen samples were pooled together and split into equal parts in Falcon tubes. Three samples were combine...

The present investigation aimed to conduct a genetic study of dairy cattle and buffalo bulls based on economic traits and their phenotypic correlation. The means of various economic traits in both the species are presented. Cattle bulls reported a better libido score, Flehmen response, requirement of mounting stimulus and semen volume, conception rate and expected predicted difference for milk ...