OBJECTIVES To describe the use of sonication and microcalorimetry in diagnosing necrotizing fasciitis in a 27-year-old woman with bilateral breast implants. METHODS The removed breast implants were subjected to sonication and microcalorimetry. The microcalorimetry findings were correlated with conventional microbiologic methods. The time to detection of infection was noted. RESULTS The pati...

BACKGROUND Culturing of the sonication fluid of removed implants has proven to be more sensitive than conventional periprosthetic tissue culture for the microbiological diagnosis of prosthetic joint infection. Since bacteria surviving on antibiotic-loaded cement spacers used in a two-stage exchange protocol for infected arthroplasties may cause the persistence of infection, in this study we ask...

BACKGROUND Transformed cells of Escherichia coli DH5-alpha with pGFPuv, induced by IPTG (isopropyl-beta-d-thiogalactopyranoside), express the green fluorescent protein (gfpuv) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-phase partitioning extraction (TPP) method were compared to the direct applica...

Developing cellulose nanocrystal (CNCs) preparation techniques is a challenge confronted by many researchers. The advantages of property remain the reason for research to be developed. To deal with this issue, it essential conduct related process optimization, particularly in hydrolysis process, which primary step forming CNCs. In study, effect sonication-assisted time was investigated. XRD cha...

BACKGROUND Liposomes have been successfully used as delivery vehicles for anticancer drugs. Both sonication and microfluidic technologies have been used to produce liposomes. The combination of the two methods was evaluated in this study. MATERIALS AND METHODS The microfluidic devices, mainly comprising micro-dispensers and a sonicator, were used to produce liposomal nanoparticles. Sonication...

Lignocellulosic wastes are used as feed stock since are abundantly available. The basic problems involved with bioethanol production from lignocellulosic wastes are high consumption of chemicals used for saccharification, delignification and glucose availability. These problems are considered as important themes of the present study. Ultrasound assisted saccharification was carried out using sa...

Many different extraction and analysis methods exist to determine the protein fraction of microbial cells. For metabolic engineering purposes it is important to have precise and accurate measurements. Therefore six different protein extraction protocols and seven protein quantification methods were tested and compared. Comparison was based on the reliability of the methods and boxplots of the n...

Many earlier studies have documented pasteurization problems in the dairy industry. As a result, ultrasonic processing has been researched as non‐heat alternative to pasteurization. In this study, milk‐based soft cheese was treated using various sonication times (0, 1, and 3 min) at set frequency (22 kHz) with an amplitude of 60% 630 W different ripening periods 15, 30, 60 days) brine (15%), st...

Existence of 2-methylpropane-2-thiol as an organosulfur and odorant compound in the soil could causes environmental problem and social dissatisfaction. In this study, remediation of this type of thiol using ultrasound is investigated. Central Composite Design (CCD) based on Response Surface Model (RSM) was used to obtain effects of the main factor (Power, sonication time and amount of water) an...

Background: Sonication fluid culture is routinely conducted in patients with suspected orthopedic device-related infection (ORI). However, its value the assessment of devices that have explanted because presumed aseptic loosening still debatable. A meta-analysis was to estimate yield sonication detecting presence microorganisms loosening, and summarize clinical characteristics outcomes these pa...