BACKGROUND Bacterial DNA contamination in PCR reagents has been a long standing problem that hampers the adoption of broad-range PCR in clinical and applied microbiology, particularly in detection of low abundance bacteria. Although several DNA decontamination protocols have been reported, they all suffer from compromised PCR efficiency or detection limits. To date, no satisfactory solution has...

introduction: knowledge is a critical resource for organizations working in dynamically competitive environments. in any organization, leaders who have the power to incite and influence knowledge management processes. nevertheless, there is lack of empirical evidence from previous studies on examining this relationship. in view of this, the current study examined the relationship between transf...

introduction: foodborne pathogens are a major public health problem. despite the increase in management and consumer interest in food safety, the number of food poisoning cases has been continuously increased in recent years. for example in 2010, human salmonellosis cases were reported in europe and in 2011, a multistate foodborne e.coli o157:h7 outbreak affected 8 people and also a larger mult...

The incidence of false positives due to the presence of bacterial DNA in Taq DNA polymerase is an obstacle to the use of PCR in the diagnosis of infection. We describe a method that uses a restriction enzyme to destroy the ability of contaminating sequences to act as templates for a nested PCR which uses primers based on the 16S rRNA genes. The method was used prior to a PCR that amplified 10 f...

The identification of methylated sites on bacterial genomic DNA would be a useful tool to study the major roles of DNA methylation in prokaryotes: distinction of self and nonself DNA, direction of post-replicative mismatch repair, control of DNA replication and cell cycle, and regulation of gene expression. Three types of methylated nucleobases are known: N6-methyladenine, 5-methylcytosine and ...