Murine monoclonal antibodies that bind the O-antigens of Yersinia enterocolitica serotype O:9 and Brucella abortus 1119-3 were generated after immunization of BALB/c mice with killed, whole cells. Highly purified lipopolysaccharide preparations from each organism were used to screen for antigen-specific antibodies. Immunization with B. abortus cells induced 56 antigen-specific hybrids, and 10 o...

The serological response profile of severe acute respiratory syndrome (SARS) coronavirus (CoV) infection was defined by neutralization tests and subclass-specific immunofluorescent (IF) tests using serial sera from 20 patients. SARS CoV total immunoglobulin (Ig) (IgG, IgA, and IgM [IgGAM]) was the first antibody to be detectable. There was no difference in time to seroconversion between the pat...

A. Introduction B. Clinical manifestations of genital herpes 1. Primary genital herpes 2. Non-primary first episodes 3. First episode reactivation 4. Recurrent genital herpes 5. Genital herpes in immunocompromised patients 6. Asymptomatic or unrecognised genital herpes 7. Genital herpes in pregnancy 8. Diagnostic testing of neonates C. Laboratory techniques to detect HSV 1. Viral culture a. Cul...

Ever since the first report of the Wassermann reaction in 1906 the physician has often been faced with the problem of correctly interpreting and coordinating with the clinical findings the results of various standard serological tests for syphilis. Improvements in the methods and materials, particularly the purification of the antigen in the form of cardio'ipin, have not completely eliminated t...

West Nile Fever is a zoonotic disease caused by a mosquito-borne flavivirus, WNV. By its clinical sensitivity to the disease, the horse is a useful sentinel of infection. Because of the virus' low-level, short-term viraemia in horses, the primary tools used to diagnose WNV are serological tests. Inter-laboratory proficiency tests (ILPTs) were held in 2010 and 2013 to evaluate WNV serological di...

results in this setting, detection rate of the disease is 34.86%, if the high normal titer for the wrights test titer assumed 1/80 and is 44.95% if the titer assumed 1/40, while elisa test had detection rate of 58.72% for those suspicious cases. furthermore, the detection rate for diagnosis of active brucellosis with 2me tests was 17.54% with high normal titer of 1/80 and was 59.64% with a high...

To improve our understanding of the prostate-specific antigen (PSA) antigenic regions, we studied the association targets of one anti-PSA polyclonal antibody and 10 anti-PSA monoclonal antibodies (mAbs). We also examined the ability of the mAbs to inhibit PSA enzymatic activity and block the association of PSA with alpha 1-antichymotrypsin (ACT). Linear epitope mapping with a polyclonal antibod...

Convenient, sensitive, and specific solid-phase immunoassays involving monoclonal antibody are described for the determination of human placental alkaline phosphatase (hPLAP). An endogenous enzyme immunoassay combined the specificity of the immunological and the enzymatic reactions. Alternatively, a solid-phase "sandwich" radioimmunoassay involving immobilized polyclonal rabbit anti-hPLAP in co...