نتایج جستجو برای: was amplified and sequenced using conventional rt
تعداد نتایج: 17375690 فیلتر نتایج به سال:
iv abstract this study examined the linguistic behaviors of two iranian efl teachers each of them teaching learners of two similar proficiency levels, a beginner level and an intermediate level, to investigate the relationship between the learners proficiency levels and the amounts and purposes for l1 use by the two teachers. the study was carried out to investigate whether there were differe...
abstract: literature is said beautiful words of poetry or prose that excites reader’s or listener’s feel. certainly, to be effective, such a text should have certain characteristics. the four elements of the literature: thought, imagination, emotion and style make a text to be literary and effective. emotion and imagination are specific elements of literary texts, while thought and style a...
The genomes of clusters of related eukaryotes are now being sequenced at an increasing rate, creating a need for accurate, low-cost annotation of exon-intron structures. In this paper, we demonstrate that reverse transcription-polymerase chain reaction (RT-PCR) and direct sequencing based on predicted gene structures satisfy this need, at least for single-celled eukaryotes. The TWINSCAN gene pr...
the effect of poss instruction in comparison with conventional instruction on development of esp learners’ parts of speech knowledge and attitude toward call.
AIM To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses. MATERIALS AND METHODS A total of 377 bursa samples were collected from 201 suspected IBD...
this research was conducted to examine the effect of a concurrent training on rest level of leptin of plasma and some hormonal factors in non-athlete subjects. the research population included non-athlete men who didn’t participate in any organized sport activities. 30 men were chosen voluntarily among the above-mentioned population and were divided into 2 groups: experimental (15 subjects) an...
background: lipophosphoglycan 3 (lpg3) is required for the lpg assembly, a well known virulent molecule. in this study, the lpg3 gene of the lizard and mammalian leishmania species were cloned and sequenced. a three-dimensional structure (3d) for the target sequence was also predicted by comparative (homology) modeling. materials and methods: an optimization pcr amplification was performed on g...
Abstract Mealybug wilt of pineapple (MWP) is the most important disease worldwide. During months September and November 2020, production fields cultivar Hawaiiana hybrid MD-2 in Satipo Chanchamayo provinces Peru, showed typical symptoms MWP. Based on symptoms, incidence level ranged between 70 90%. Symptomatic asymptomatic foliar samples were collected tested by RT-PCR using primers specific fo...
PURPOSE To determine whether NOX 5 is expressed in rabbit corneal stromal cells (RCSC). NADPH oxidases (NOXes) are enzymes that preferentially use NADPH as a substrate and generate superoxide. Several isoforms of NOXes function as multi-protein complexes while NOX5 and DUOXs do not require the accessory proteins for their activity and possess calcium binding EF hands. METHODS Human NOX5 prime...
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