background hepatocellular carcinoma (hcc), a major fatal cancer worldwide, is induced by different etiological factors in the liver. objectives to gain insight into serum protein profiling of hcc with different etiologies. patients and methods we subjected the sera of hbv-hcc, hcv-hcc, non-b non-c-hcc patients, and healthy volunteers to two-dimensional gel electrophoresis (2-de) and liquid chro...

conclusions these results show the usefulness of proteome analysis in successful identification of the common proteins between n. lactamica strains and nmb. this proteomics analysis is the starting point in the path of knowledge development about whole proteome profiles of n. lactamica strains. objectives the main aims of this study were to determine whole proteome profiles of n. lactamica stra...

In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-base...

The automated interpretation of two-dimensional gel electrophoresis images used in protein separation and analysis presents a formidable problem in the detection and characterization of ill-defined spatial objects. We describe in this paper a hierarchical algorithm that provides a robust, high-sensitivity solution to this problem, which can be easily adapted to a variety of experimental situati...

background: in this study, two-dimensional gel electrophoresis (2-de) method was applied to determine and compare the protein spots expressed in the two field isolates of leishmania major and recovered from the patients who were clinically sensitive and resistant to glucantime® treatment. methods: leishmania parasites were isolated from the cutaneous lesions of two cl infected patients in shira...

Two-dimensional gel electrophoresis (2-DE) separation has not been considered suitable for large-scale comparative protein expression studies due to its limited throughput. We present a high-throughput analysis method based on three-dimensional (3-D) geometry gel electrophoresis. Following conventional isoelectric focusing (IEF), up to 36 immobilized pH gradient (IPG) strips are arrayed on the ...