Alginate is a linear and acidic polysaccharide, composed of (1 → 4) linked β-D-mannuronic acid (ManA) and α-L-guluronic acid (GulA). The ratio of ManA to GulA (M/G) is one of the most important factors for the application of alginate and its derivatives in various areas. In this work, a robust and accurate method was developed to analyze M/G using high-performance anion-exchange chromatography ...

BACKGROUND The current study was designed to determine if follitropin alfa (recombinant human follicle-stimulating hormone; r-hFSH) and lutropin alfa (recombinant human luteinizing hormone; r-hLH) biopotencies were unchanged by reconstituting in sterile water for injection and mixing prior to injection. METHODS The biopotencies of r-hFSH and r-hLH were determined following injection of female...

Background Alginate is a polysaccharide constituted by β-d-mannuronic acid and α-l-guluronic which has several applications in the field of tissue engineering, drug delivery other biomedical applications. This study aimed to evaluate production, molecular weight (MW) viscosifying power alginate produced strain AT9 cultured 3-L reactor, under three different oxygen transfer rates (OTR). Results ...

A characteristic feature of type I allergies is the presence of allergen-specifi c immunoglobulin E (sIgE) [1,2]. Thus, the detection of sIgE, in addition to obtaining a clinical history and performing a skin prick test (SPT), is important for allergy workup. Historically, sIgE to various allergens was analyzed by radioallergosorbent test using allergen-coupled cellulose paper discs. Later on, ...

Mannuronan C-5 epimerases (ManC5-Es) are produced by brown algae and some bacteria, such as Azotobacter Pseudomonas species. It can convert the transformation of β-D-mannuronic acid (M) to α-L-guluronic (G) in alginate with different patterns epimerization. Alginate compositions monomer sequences possess properties functions, which have been utilized industries for various purposes. Therefore, ...

Alginate is an industrially important polysaccharide obtained commercially by harvesting brown algae. The final step in alginate biosynthesis, the epimerization of beta-1,4-d-mannuronic acid to alpha-1,4-l-guluronic acid, a structural change that controls the physicochemical properties of the alginate, is catalyzed by the enzyme mannuronan C-5-epimerase. Six different cDNAs with homology to bac...

Alginic acid gels were studied by small-angle X-ray scattering and rheology to elucidate the influence of alginate chemical composition and molecular weight on the gel elasticity and molecular structure. The alginic acid gels were prepared by homogeneous pH reduction throughout the sample. Three alginates with different chemical composition and sequence, and two to three different molecular wei...

All the experiments in the paper were conducted using these materials : CdO (99.999%, Strem Chemicals, MA, USA), Se (99.999%, Alfa-Aesar, MA, USA), trioctylphosphine (TOP; 97%, SigmaAldrich, Israel), trioctylphosphine oxide (TOPO; 99%, Sigma-Aldrich, Israel), octadecylphosphonic acid (ODPA; 99%, Polycarbon Industries, MA, USA), S (99.98%, Sigma-Aldrich, Israel), oleic acid (90%, Alfa-Aesar, MA,...