Background To assess embryos derived by the transfer of meiosis-II chromosomes (M-II-t) from aged mice oocytes into ooplasms from younger mice to overcome the problem of age-related decline in female fertility. MaterialsAndMethods The developmental capacity karyotype and ultrastructure of reconstructed oocytes derived from meiosis-II chromosome transplantation from aged mice into the ooplasms o...

Background The purpose was to investigate the effect of the duration of gamete incubation on fertilization rate, embryo cleavage, and embryo quality before and after freezing in mice. MaterialsAndMethods Ovulated oocytes collected from superovulated mice after ip injection of PMSG and hCG were divided randomly into control and experimental groups. Oocytes from the control group were inseminated...

Gap detection or gap pre-pulse inhibition of the acoustic startle (GPIAS) has been successfully used in rat and guinea pig models of tinnitus, yet this system has been proven to have low efficacy in CBA mice, with low basal GPIAS and subtle tinnitus-like effects. Here, we tested five mouse strains (CBA, BalbC, CD-1, C57BL/6 and 129sv) for pre-pulse inhibition (PPI) and gap detection with varyin...

Novel therapies for lung cancer are being explored nowadays with local therapies being the tip of the arrow. Intratumoral chemotherapy administration and local microwave ablation have been investigated in several studies. It has been previously proposed that lipiodol has the ability to modify the microenvironment matrix. In our current study we investigated this theory in BALBC mice. In total 1...

Lung cancer remains the leading cause of death in cancer patients. Severe treatment side effects and late stage of disease at diagnosis continue to be an issue. We investigated whether local treatment using 2-diethylaminoethyl-dextran methyl methacrylate copolymer with p53 (DDMC-p53) with or without cisplatin and/or microwave ablation enhances disease control in BALBC mice. We used a Lewis lung...

Diarrhea is a disease that still health problem worldwide; one of the causes Shigella flexneri. Non-dairy probiotic functional foods and beverages are gaining popularity, with researchers seeking to assuage consumer concerns regarding lactose intolerance veganism. Coffee has anti-inflammatory antibacterial properties. This study aims determine effect mixture Lampung Robusta coffee extract Lacto...

introduction: to improve the development of embryo in vitro several attempts have been made, including the application of leukemia inhibitory factor (lif). this factor plays an important role in embryo development and has been shown to enhance in vitro development rate in sheep and mice. there is controversy regarding the effect of lif on the different stages of embryo development. the aim of t...

Background: The present study has been carried out to investigate the effects of preovulatory stage gamma-irradiation of female mice in the absence or presence of vitamin E on numerical chromosome abnormalities in 8-cell embryos after mating with non-irradiated males. Materials and Methods: The 8-11 weeks adult female NMRI mice were whole body irradiated at preovulatory stage (post PMSG inject...

Introduction: HESA-A is an active natural compound with herbal and marine origin. It contains inorganic, organic and aqueous fractions, and has shown antioxidant, cytotoxic and anticancer effects. In this study, the teratogenic effects of HESA-A in mice have been evaluated. Methods: Several doses of HESA-A were administered orally to pregnant mice on days 6 to 14 of gestation. Various parameter...

vitex agnus castus (chasteberry) is a popular treatment for the management of female reproductive disorders including corpus luteum insufficiency, premenstrual syndrome (pms), menopausal symptoms, and insufficient milk production. according to developing situation of complementary medicine, and frequent use of this herb, it is important to examine its effects during pregnancy. in this research ...