مزدارانی, حسین
[ 1 ] - فراوانی آسیبهای کروموزومی ناشی از داروی بلئومایسین سولفات در سلولهای هیبریدومای F3B6 و HF2x653 در مقایسه با سلولهای غیرهیبرید والدی
Background and Objective: Hybrid cells are made from fusion of two or more somatic cells. After formation chromosomes are located in one membrane . . So nucleic condition of each fused cell has changes and two genomes and chromosomes interact with each other. Locating the genes in new nucleic and cytoplasmic condition and great chromosomal rearrangement in these new formed cells can affect th...
[ 3 ] - Impact of DNA Damage on the Frequency of Sperm Chromosomal Aneuploidy in Normal and Subfertile Men
Background: Various frequencies of sperm aneuploidy are reported in sperms of subfertile patients compared to normal individuals. Moreover, sperm DNA damage is shown to be associated with male infertility. In this study, the rate of DNA damage and frequencies of aneuploidy in sperms of subfertile patients was investigated. Methods: Semen samples were obtained from healthy normal and subfertile ...
[ 4 ] - Radiosensitivity and Repair Kinetics of Gamma-Irradiated Leukocytes from Sporadic Prostate Cancer Patients and Healthy Individuals Assessed by Alkaline Comet Assay
Background: Impaired DNA repair mechanism is one of the main causes of tumor genesis. Study of intrinsic radiosensitivity of cancer patients in a non-target tissue (e.g. peripheral blood) might show the extent of DNA repair deficiency of cells in affected individuals and might be used a predictor of cancer predisposition. Methods: Initial radiation-induced DNA damage (ratio of Tail DNA/Head DN...
[ 5 ] - DNA Damage in Leukocytes from Fanconi Anemia (FA) Patients and Heterozygotes Induced by Mitomycin C and Ionizing Radiation as Assessed by the Comet and Comet-FISH Assay
Background: Lymphocytes of Fanconi anemia (FA) show an increased sensitivity to the alkylating agents such as mitomycin C (MMC), but their responses to gamma-irradiation is controversial. The extent of DNA damage in leukocytes of FA patients following irradiation and MMC treatment was studied at cellular and single chromosome level. Methods: DNA damage induced by gamma-rays and MMC was measure...
[ 6 ] - Expansion of Non-Enriched Cord Blood Stem/Progenitor Cells CD34+ CD38- Using Liver Cells
Many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. The proliferation and expansion of human hematopoietic stem cells in ex vivo culture were examined with the goal of generating a suitable protocol for expanding hematopoietic stem cells for patient transplantation. Using primary fetal liver cells, we established...
[ 7 ] - Editorial Report of fifteen years contributions to radiological sciences: Future directions and prospects
At the close of the 20th century, fundamental discoveries changed broadly the worlds of physics, biology and medicine. The rapid advancements achieved during recent years, mainly due to revolutionary methodological improvements, have led to an unparalleled explosion of information; often appear to overshadow the earlier works. However as more basic discoveries are made these separate scientific...
[ 8 ] - Quantitative evaluation of abscopal effect based on biological effective dose in breast cancer tumors in mice
Background: Due to abscopal effect, cell damage may occur outside of the radiation field and the quantification of this effect is one of the most challenging debates in radiation therapy. The aim of this study was to estimate the abscopal effect induced in non-irradiated tumors quantitatively by means of biological effective dose (BED). Materials and Methods: Breast tumors using 4T1 and MC4-L2 ...
[ 9 ] - Inherent radiosensitivity and its impact on breast cancer chemo-radiotherapy
About 10% of apparently normal individuals are sensitive to clastogenic effects of physico-chemical agents. More than 45% of breast cancer patients’ exhibit elevated radiosensitivity. Although the nature of inherent radiosensitivity is not fully understood, but insufficiency and impaired DNA repair mechanism might be prime cause of radiosensitivity. This is evident from genetically affect...
[ 10 ] - Induction of DNA damage, apoptosis and micronuclei in peripheral blood lymphocytes following injection of contrast media in patients with abdominal CT scan
Background: Medical diagnostic procedures such as X-ray and computed Tomography (CT) scan account for considerable percent of patient's exposure to ionizing radiation. The exposure of cells to Ionization radiation results in induction of DNA damage and chromosomal aberrations. Contrast media (CM) are widely used in diagnostic radiology and CT scan. The aim of this study was to study adverse gen...
[ 11 ] - Potent radioprotective effect of herbal immunomodulator drug (IMOD) on mouse bone marrow erythrocytes as assayed with the micronucleus test
Background: Although numerous natural or synthetic drugs have been tested for their radioprotective capacity, yet no suitable drug has been introduced for routine clinical use. In this study the radioprotective effect of "a new herbal immunomodulator" commercially known as IMOD, specifically made to decrease the side effects of HIV virus, was investigated on mouse bone marrow cells. M...
[ 12 ] - Can recent Berkeley findings help us to find a solution to the paradox of cancer incidence in high natural background radiation areas of Ramsar, Iran?
According to the report published by the United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR) in 2000, Ramsar city in northern Iran, has some inhabited areas with the highest known natural background radiation levels in the world (1). The annual radiation absorbed dose in high background radiation areas (HBRAs) of Ramsar is extraordinary high, reaching 260 mSv that i...
[ 13 ] - Radio-adaptive response of peripheral blood lymphocytes following bystander effects induced by preirradiated CHO-K1 cells using the micronucleus assay
Background: Radio-adaptive response and bystander effects are known phenomena occurring in cells following exposure to ionizing radiation (IR). In this study we examined possible radio-adaptation of lymphocytes following bystander effects induced by CHO-K1 cells. Materials and Methods: Whole blood and CHO-K1 cells were cultured in RPMI-1640 complete medium. Cells were separately irradiated with...
[ 14 ] - γ-H2AX as a protein biomarker for radiation exposure response in ductal carcinoma breast tumors: Experimental evidence and literature review
Background: H2AX is a histone variant that is systematically found and ubiquitously distributed throughout the genome. DNA double-strand breaks (DSBs) induce phosphorylation of H2AX at serine 139 (γH2AX), an immunocytochemical assay with antibodies recognizing γH2AX has become the gold standard for the detection of DSBs. The importance of this assay to investigate different individu...
[ 15 ] - Deep space missions and the issue of overcoming the problem of space radiation
As a member of the United Nations Committee on the Peaceful Uses of Outer Space (COPUOS), Iran has a long-term space exploration program. Space radiation is one of the challenges facing humans when they go outside Earth's protective atmosphere and magnetic field. Space is an environment that the cardinal principles of radiation protection i.e. time, distance and shielding cannot be effectively ...
[ 16 ] - Radioadaptive response in peripheral blood leukocytes of occupationally exposed medical staff with investigation of DNA damage by the use of neutral comet assay
Background: ˝Radioadaptive Response˝ is well-documented phenomenon appeared in low dose ionizing radiation received in vitro and in vivo. Occupational exposure has always been a great concern for radiation workers therefore this study was performed to study radioadaptive response in terms of residual DNA double strand breaks as an endpoint in peripheral blood leukocytes of occupationally expose...
[ 17 ] - Uncertainties in radiation sciences: The need for more researches and publications. Experience of 10 years publications in this field and our new plans
Three nearly simultaneous events around the end of the 19th century, i.e., the discovery of X‐rays by Roentgen in 1895, natural radioactivity by Antoine‐Henri Becquerel in 1896 and isolation of Radium by the Curies' in 1898 opened the nuclear era which has changed broadly the worlds of physics, biology and medicine. At the close of the 20th century, fundamental discoveries in biology at molecul...
[ 18 ] - Radiosensitizing effects of gemcitabine on aerobic and chronically hypoxic HeLa and MRC5 cells in-vitro
Background: Gemcitabine (2′, 2′-difluoro-2′- deoxycytidine, an analogue of deoxycytidine) is a relatively new drug with wide range of anti-cancer activity. In this study, radiosensitizing effects of gemcitabine was investigated on HeLa and MRC5 human originated cell lines under both chronically hypoxic and normoxic conditions using the micronucleus (MN) assay. Materials and Methods: F...
[ 19 ] - G2 chromosomal radiosensitivity and background frequency of sister chromatid exchanges of peripheral blood lymphocytes of breast cancer patients
Background: Chromosomal alterations play an important role in carcinogenesis. Enhanced chromosomal radiosensitivity is shown for many cancer predisposition conditions including breast cancer. In this study chromosomal radiosensitivity and the frequency of background sister chromnatid exchanges (SCE) in lymphocytes of normal individuals and breast cancer patients was compared. Materials...
[ 20 ] - Protective effects of famotidine and vitamin C against radiation induced cellular damage in mouse spermatogenesis process
Background: Radioprotective effect of famotidine was previously shown on radiation induced micronuclei and chromosomal aberrations in human peripheral lymphocytes and mouse bone marrow cells however, its radioprotective property has never been studied in mouse spermatogenesis. It was also shown that vitamin C as an antioxidant also exert its radioprotective effect on many biological s...
[ 21 ] - Induction of chromosomal aberrations in human primary fibroblasts and immortalized cancer cells exposed to extremely-low-frequency electromagnetic fields
Background: Rapidly increasing possibilities of exposure to environmental extremely low-frequency electromagnetic fields (ELF-EMF) have become a topic of worldwide investigation. Epidemiological and laboratory studies suggest that exposure to ELF-EMF may increase cancer risk therefore assessment of chromosomal damage in various cell lines might be of predictive value for future risk es...
[ 22 ] - Modification of 10 cGy neutron or gamma-rays induced chromosomal damages by hyperthermia: an in vitro study
Background: To evaluate the effects of hyperthermia (HT) on the frequency of chromosomal aberrations induced by a low dose of neutron or γ-rays in human peripheral blood lymphocytes. Materials and Methods: Blood samples were exposed to HT (41.5°C for 30 and 60min, 43°C for 15 and 30min), 10 cGy neutron or γ-rays, HT + neutron/γ, and neutron/γ + HT. After standard cell culture, harvestin...
[ 23 ] - Effect of cimetidine and famotidine on survival of lethally gamma irradiated mice
Background: Currently available radioprotectors are poorly tolerated in man and the general use of aminothiols is compromised by their side effects. This study was carried out to test and compare the radioprotective potential of cimetidine and famotidine against lethally gamma irradiated NMRI mice. Materials and Methods: Adult male NMRI mice in groups of 10 were exposed to various doses of gamm...
[ 24 ] - Assessment of the radioprotective effects of amifostine on human lymphocytes irradiated in vitro by gamma-rays using cytokinesis-blocked micronucleus assay
Background: A radioprotective effect of amifostine as well as its ability to modulate the level of spontaneous and gamma-irradiation-induced genetic changes on human peripheral blood lymphocytes has been investigated . Amifostine, known as a potent radical scavenger, has been introduced as the most effective radioprotector, yet it is not completely approved for the clinical use. However, furt...
[ 25 ] - Effect of vitamin E on preovulatory stage irradiated female mouse expressed as chromosomal abnormalities in generated embryos
Background: The present study has been carried out to investigate the effects of preovulatory stage gamma-irradiation of female mice in the absence or presence of vitamin E on numerical chromosome abnormalities in 8-cell embryos after mating with non-irradiated males. Materials and Methods: The 8-11 weeks adult female NMRI mice were whole body irradiated at preovulatory stage (post PMSG inject...
[ 26 ] - Being on the right path
Being on the right pathThe 1st issue of the Iranian Journal of Radiation Research (IJRR) was published two years ago (June 2003). This journal was initiated to bring together the various disciplines of radiation oncology, radiation biology, medical physics, nuclear medicine and other related subjects to intensify the dialogue between basic and clinical researchers especially those working in Ir...
[ 27 ] - Effect of famotidine on radiation induced apoptosis in human peripheral blood leukocytes
ABSTRACTBackground: Radioprotective effects of famotidine, an antagonist of H2 receptor clinically used for peptic ulcer treatment, was previously shown on radiation-induced micronuclei and chromosomal aberration in human peripheral blood lymphocytes and mouse bone marrow cells. This study was conducted to investigate radioprotective property of famotidine against radiation induced apoptosis i...
[ 28 ] - Potent radioprotective effect of therapeutic doses of ranitidine and famotidine against gamma-rays induced micronuclei in vivo
Background: Previous investigations have revealed, cimetidine, a histamine H2-receptor antagonist, show radioprotective effects against gamma- and neutron-induced micronuclei in bone marrow erythrocytes. In this study, the anticlastogenic effects of famotidine and ranitidine, which act similar to cimetidine as histamine H2-receptor antagonists, was investigated. Materials and Methods : Balb/c m...
[ 29 ] - آسیب های زمینه DNA در اسپرم های افراد سالم و نابارور با استفاده از روش سنجش ستاره دنباله دار
مقدمه: ناباروری در مردان، عامل 30% ناباروری زوجین در بین دلایل دیگر می باشد. اختلال در مواد ژنتیکی حایز اهمیت است زیرا موجب انتقال ناقص اطلاعات وراثتی به جنین می شود . به همین دلیل ، حفظ تمامیت و صحت DNA درهسته اسپرم، برای انتقال تمام و کمال ماده وراثتی از نسلی به نسل دیگر بسیار مهم است. هدف از این تحقیق ارزیابی و مقایسه میزان آسیب زمینه در DNA اسپرم چهار گروه افراد...
[ 30 ] - ارزیابی آسیبهای کروموزومی بیماران مبتلا به سرطان مری پیش و پس از رادیوتراپی با استفاده از روش آزمون میکرونوکلئی
Background: Radiotherapy can cause DNA damage in normal cells, misrepaired or unrepaired double strand breaks in DNA lead to chromosomal breaks. As a result patient experience early and late effects in normal tissue during and after radiotherapy. Cytogenetic techniques can be used as a cancer predictive assay because there is an association between chromosome abnormalities and the risk of devel...
[ 31 ] - بررسی تاثیر هیپوکسی بر تغییرات بیان 107miR- در رده های سلولی سرطان معده 45MKN و AGS
سابقه و هدف: هیپوکسی در تومورهای جامد، علت اصلی مقاومت به درمان در سرطان است. بنابراین شناخت اندیکاتورهای مناسب جهت آگاهی از وضعیت هیپوکسی در تومور برای انجام روش های صحیح درمانی و آگاهی از پیش اگهی تومور بسیار مفید و ضروری است. هدف از انجام این تحقیق بررسی تغییر بیان miR-107 به عنوان بیومارکر و اندیکاتور هیپوکسی و ارتباط آن با هیپوکسی در سلول های سرطان معده می باشد. مواد و روشها: در این تح...
[ 32 ] - Protection Against Radiation-Induced Micronuclei in Rat Bone Marrow Erythrocytes by Curcumin and Selenium L-Methionine
Background: The search for potent radioprotective agents for the amelioration of radiation side effect is an important aim in radiobiology. The present study aimed to evaluate the effects of curcumin and seleno-L-methionine against radiation-induced micronucleus formation in rat bone marrow.Methods: In total, 40 male rats were divided into 8 groups (n=5 each), including control, curcumin or sel...
[ 33 ] - In Vitro Radiosensitizing Effects of Temozolomide on U87MG Cell Lines of Human Glioblastoma Multiforme
Background: Glioma is the most common primary brain tumor with poor prognosis. Temozolomide (TMZ) has been used with irradiation (IR) to treat gliomas. The aim of the present study was to evaluate the cytotoxic and radiosensitizing effect of TMZ when combined with high-dose and high-dose rate of gamma irradiation in vitro.Methods: Two ‘U87MG’ cell lines and skin fibroblast were cultured and ass...
[ 34 ] - Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and It's Definite Dose Reduction Factor
Background: Interactions of free radicals from ionizing radiation with DNA can induce DNA damage and lead to mutagenesis and carsinogenesis. With respect to radiation damage to human, it is important to protect humans from side effects induced by ionizing radiation.In the present study, the effects of arbutin were investigated by using the micronucleus test for anti-clastogenic activity, to cal...
[ 35 ] - Cellular dosimetry of beta emitting radionuclides-antibody conjugates for radioimmunotherapy
Introduction: The choice of optimal radionuclides for radioimmunotherapy depends on several factors, especially the radionuclide and antibody. The dosimetric characteristics of a non-internalizing and an internalizing monoclonal antibody (MAb) labeled with beta emitting radionuclides were investigated. Methods: Using Geant4-DNA Monte Carlo simulation, we ...
[ 36 ] - Sperm DNA damage in mice irradiated with various doses of X-rays alone or in combination with actinomycin D or bleomycin sulfate: an in vivo study
Background: DNA damage in male germ cells due to exposure to environmental and manmade physico-chemical genotoxic agents is considered as the main cause of male infertility. The aim of this study was to evaluate the effects of combined modalities (radiotherapy and chemotherapy) routinely used for cancer treatment on mouse sperm chromatin in vivo. Materials and Methods: Forty-eight mice were div...
[ 37 ] - The Cell Survival of F10B16 Melanoma and 4T1 Breast Adenocarcinoma Irradiated to Gamma Radiation Using the MTT Assay Based on Two Different Calculation Methods
Background: Various MTT assay methods are proposed to obtain the cell sur- vival parameters.Objective: Determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel MTT assay method after exposing them to ionizing radiation.Method: A common and a novel MTT assay method were used and compared for obtaining the F10B16 melanoma and 4T1 breast adeno...
[ 38 ] - Radiosensitizing effects of melatonin on radiation induced chromosomal aberration in G2-lymphocytes of breast cancer patients
Background: Radiotherapy is regarded as a standard treatment modality in breast cancer (BC). Radiation causes cellular damage both in cancer and normal cells by inducing DNA-damage and chromosomal aberrations (CA). Different agents were used for ameliorating effects of radiation, mainly antioxidants such as melatonin. Melatonin shows oncostatic properties on human BC. The aim of this study was ...
[ 39 ] - ACKNOWLEGMENT
Many scientists and colleagues from abroad and Iran (listed below) voluntarily helped us very much in review and evaluation process of manuscripts being accepted for publication in 2020 issues of IJRR (Volume 18, numbers 1-4) and those not suitable for publication. Indeed their careful and critically reading the manuscripts and their valuable comments and suggestions greatly improved the qualit...
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